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International journal of molecular sciences
Jul 14, 2016;17 (7):

Dermal Lipogenesis Inhibits Adiponectin Production in Human Dermal Fibroblasts while Exogenous Adiponectin Administration Prevents against UVA-Induced Dermal Matrix Degradation in Human Skin.

Chien-Liang Fang, Ling-Hung Huang, Hung-Yueh Tsai, Hsin-I Chang
Author Information
  1. Chien-Liang Fang: Division of Plastic and Reconstructive Surgery, Department of Surgery, Ditmanson Medical Foundation Chia-Yi Christian Hospital, No. 539, Zhongxiao Rd., Chiayi City 60002, Taiwan. fangcl68@hotmail.com.
  2. Ling-Hung Huang: Department of Biochemical Science and Technology, National Chia Yi University, No. 300, Syuefu Rd., Chiayi City 60004, Taiwan. s102605@alumni.ncyu.edu.tw.
  3. Hung-Yueh Tsai: Department of Biochemical Science and Technology, National Chia Yi University, No. 300, Syuefu Rd., Chiayi City 60004, Taiwan. s1040681@mail.ncyu.edu.tw.
  4. Hsin-I Chang: Department of Biochemical Science and Technology, National Chia Yi University, No. 300, Syuefu Rd., Chiayi City 60004, Taiwan. hchang@mail.ncyu.edu.tw.
PMID: 27428951 DOI: 10.3390/ijms17071129

Abstract

Adiponectin is one of the most abundant adipokines from the subcutaneous fat, and regulates multiple activities through endocrine, paracrine, or autocrine mechanisms. However, its expression in adipogenic induced fibroblasts, and the potential role in photoaging has not been determined. Here, human dermal fibroblasts, Hs68, were presented as a cell model of dermal lipogenesis through stimulation of adipogenic differentiation medium (ADM). Similar to other studies in murine pre-adipocyte models (i.e., 3T3-L1), Hs68 fibroblasts showed a tendency to lipogenesis based on lipid accumulation, triglyceride formation, and the expressions of PPAR-γ, lipoprotein lipase (LPL), and FABP4 mRNA. As expected, ADM-treated fibroblasts displayed a reduction on adiponectin expression. Next, we emphasized the photoprotective effects of adiponectin against UVA-induced damage in Hs68 fibroblasts. UVA radiation can downregulate cell adhesion strength and elastic modulus of Hs68 fibroblasts. Moreover, UVA radiation could induce the mRNA expressions of epidermal growth factor receptor (EGFR), adiponectin receptor 1 (AdipoR1), matrix metalloproteinase-1 (MMP-1), MMP-3, and cyclooxygenase-2 (COX-2), but downregulate the mRNA expressions of type I and type III collagen. On the other hand, post-treatment of adiponectin can partially overcome UVA-induced reduction in the cell adhesion strength of Hs68 fibroblasts through the activation of AdipoR1 and the suppression of EGF-R. In addition, post-treatment of adiponectin indicated the increase of type III collagen and elastin mRNA expression and the decrease of MMP-1 and MMP-3 mRNA expression, but a limited degree of recovery of elastic modulus on UVA-irradiated Hs68 fibroblasts. Overall, these results suggest that dermal lipogenesis may inhibit the expression of adiponectin while exogenous adiponectin administration prevents against UVA-induced dermal matrix degradation in Hs68 fibroblasts.

Keywords

adipogenic differentiation medium adiponectin human dermal fibroblast lipogenesis photoaging

References

  1. J Cell Sci. 2000 May;113 ( Pt 10):1677-86 [PMID: 10769199]
  2. J Clin Invest. 2000 Sep;106(5):663-70 [PMID: 10974019]
  3. Curr Probl Dermatol. 2001;29:83-94 [PMID: 11225204]
  4. Br J Dermatol. 2001 Feb;144(2):244-53 [PMID: 11251554]
  5. Ageing Res Rev. 2002 Sep;1(4):705-20 [PMID: 12208239]
  6. Trends Mol Med. 2002 Sep;8(9):442-7 [PMID: 12223316]
  7. J Intern Med. 2005 Feb;257(2):167-75 [PMID: 15656875]
  8. J Allergy Clin Immunol. 2005 May;115(5):911-9; quiz 920 [PMID: 15867843]
  9. Am J Clin Nutr. 2006 Feb;83(2):461S-465S [PMID: 16470013]
  10. Cancer Res. 2007 Jan 15;67(2):727-34 [PMID: 17234784]
  11. Clin Chim Acta. 2007 May 1;380(1-2):24-30 [PMID: 17343838]
  12. Osteoarthritis Cartilage. 2008 Apr;16(4):480-8 [PMID: 17869545]
  13. Cell Stem Cell. 2007 Oct 11;1(4):470-8 [PMID: 18371382]
  14. Biochem Biophys Res Commun. 2008 Oct 3;374(4):609-13 [PMID: 18656442]
  15. Exp Dermatol. 2010 Oct;19(10):878-82 [PMID: 19758317]
  16. Biochem Biophys Res Commun. 2010 Mar 12;393(3):519-25 [PMID: 20152812]
  17. Biomed Microdevices. 2010 Jun;12(3):443-55 [PMID: 20213215]
  18. Stem Cells Dev. 2011 Oct;20(10):1793-804 [PMID: 21526925]
  19. Comp Biochem Physiol A Mol Integr Physiol. 2012 Jan;161(1):83-8 [PMID: 21967882]
  20. Arthritis Res Ther. 2011;13(6):R184 [PMID: 22077999]
  21. Biochimie. 2012 Oct;94(10):2082-8 [PMID: 22750129]
  22. Sensors (Basel). 2012;12(5):5951-65 [PMID: 22778624]
  23. J Immunol. 2012 Sep 15;189(6):3231-41 [PMID: 22904306]
  24. Differentiation. 2012 Nov;84(4):305-13 [PMID: 23023066]
  25. FASEB J. 2013 Jan;27(1):277-87 [PMID: 23047894]
  26. Development. 2013 Jan 15;140(2):255-65 [PMID: 23250203]
  27. Cell Metab. 2013 Feb 5;17(2):185-96 [PMID: 23352188]
  28. Dermatoendocrinol. 2012 Jul 1;4(3):308-19 [PMID: 23467476]
  29. Exp Dermatol. 2013 May;22(5):336-41 [PMID: 23614740]
  30. ISRN Dermatol. 2013 Jun 25;2013:848705 [PMID: 23878744]
  31. J Photochem Photobiol B. 2013 Sep 5;126:42-6 [PMID: 23892189]
  32. J Dermatol Sci. 2014 Dec;76(3):196-205 [PMID: 25455138]
  33. J Dermatol Sci. 2015 Mar;77(3):173-81 [PMID: 25703056]
  34. PLoS One. 2015 Apr 24;10(4):e0121049 [PMID: 25910275]
  35. Nat Commun. 2015 Jul 15;6:7687 [PMID: 26173479]
  36. Sci Rep. 2016 May 10;6:25616 [PMID: 27161953]
  37. Toxicol Rep. 2014 Aug 29;1:658-666 [PMID: 28962279]

MeSH Term

Adiponectin
Apoptosis
Blotting, Western
Cell Adhesion
Cell Proliferation
Cells, Cultured
Fibroblasts
Fluorescent Antibody Technique
Humans
Lipogenesis
RNA, Messenger
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Skin
Skin Diseases
Ultraviolet Rays

Chemicals

ADIPOQ protein, human
Adiponectin
RNA, Messenger
Journal Article

Word Cloud

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