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Biomedical optics express
Jul 01, 2016;7 (7): 2738-48.

Alterations of filopodia by near infrared photoimmunotherapy: evaluation with 3D low-coherent quantitative phase microscopy.

Yuko Nakamura, Tadanobu Nagaya, Kazuhide Sato, Toshiko Harada, Shuhei Okuyama, Peter L Choyke, Toyohiko Yamauchi, Hisataka Kobayashi
Author Information
  1. Yuko Nakamura: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, 10 Center Dr. Bethesda,MD 20892, USA.
  2. Tadanobu Nagaya: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, 10 Center Dr. Bethesda,MD 20892, USA.
  3. Kazuhide Sato: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, 10 Center Dr. Bethesda,MD 20892, USA.
  4. Toshiko Harada: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, 10 Center Dr. Bethesda,MD 20892, USA.
  5. Shuhei Okuyama: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, 10 Center Dr. Bethesda,MD 20892, USA.
  6. Peter L Choyke: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, 10 Center Dr. Bethesda,MD 20892, USA.
  7. Toyohiko Yamauchi: Central Research Laboratory, Hamamatsu Photonics K.K., 5000, Hirakuchi, Hamakita-ku, Hamamatsu 434-8601, Japan.
  8. Hisataka Kobayashi: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, 10 Center Dr. Bethesda,MD 20892, USA.
PMID: 27446702 DOI: 10.1364/BOE.7.002738

Abstract

Filopodia are highly organized cellular membrane structures that facilitate intercellular communication. Near infrared photoimmunotherapy (NIR-PIT) is a newly developed cancer treatment that causes necrotic cell death. Three-dimensional low-coherent quantitative phase microscopy (3D LC-QPM) is based on a newly established low-coherent interference microscope designed to obtain serial topographic images of the cellular membrane. Herein, we report rapid involution of filopodia after NIR-PIT using 3D LC-QPM. For 3T3/HER2 cells, the number of filopodia decreased immediately after treatment with significant differences. Volume and relative height of 3T3/HER2 cells increased immediately after NIR light exposure, but significant differences were not observed. Thus, disappearance of filopodia, evaluated by 3D LC-QPM, is an early indicator of cell membrane damage after NIR-PIT.

Keywords

(110.6880) Three-dimensional image acquisition (110.6915) Time imaging (170.3880) Medical and biological imaging

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Journal Article

Word Cloud

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