OpenLB
Open Library of Bioscience
Advanced Search
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
Oct, 2016;37 (10): 13777-13788.

Neuropilin-1 (NRP-1)/GIPC1 pathway mediates glioma progression.

Guilong Zhang, Lukui Chen, Kouhong Sun, Ahsan Ali Khan, Jianghua Yan, Hongyi Liu, Ailin Lu, Ning Gu
Author Information
  1. Guilong Zhang: Department of Neurosurgery, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 210009, China.
  2. Lukui Chen: Department of Neurosurgery, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 210009, China. neuro_clk@hotmail.com.
  3. Kouhong Sun: Nanjing Zoonbio Biotechnology, Nanjing, 210014, China.
  4. Ahsan Ali Khan: Department of Neurosurgery, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 210009, China.
  5. Jianghua Yan: Cancer Research Center, Xiamen University, Xiamen, 361000, China.
  6. Hongyi Liu: Department of Neurosurgery, Nanjing Brain Hospital, Nanjing, 210029, China.
  7. Ailin Lu: Department of Neurosurgery, Jiangsu Province Hospital, Nanjing, 210029, China.
  8. Ning Gu: School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210009, China. guning@seu.edu.cn.
PMID: 27481513 DOI: 10.1007/s13277-016-5138-3

Abstract

Glioma occurs due to multi-gene abnormalities. Neuropilin-1 (NRP-1), as a transmembrane protein, involves in Glioma proliferation, invasion, and migration, as well as tumor angiogenesis. The cytoplasmic protein, GAIP/RGS19-interacting protein (GIPC1), could regulate the clathrin-vesicles trafficking and recycling. Here, we show that NRP-1 co-localizes and co-immunoprecipitates with GIPC1, and the C-terminal SEA-COOH motif of NRP-1 interacts specially with the named from three proteins: PSD-95 (a 95 kDa protein involved in signaling at the post-synaptic density), DLG (the Drosophila melanogaster Discs Large protein) and ZO-1 (the zonula occludens 1 protein involved in maintenance of epithelial polarity) (PDZ) domain of GIPC1 in Glioma cells. Knockdown of GIPC1 by small interfering RNA (siRNA) significantly reduces the proliferation and invasion of Glioma cells in vitro and increases its apoptosis. Furthermore, si-GIPC1 prevents the action of adaptor proteins adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1) and p130Cas and inhibits the downstream kirsten rat sarcoma viral oncogene homolog (KRAS)-ERK signaling pathway. This study demonstrated that NRP-1/GIPC1 pathway plays a vital role in Glioma progression, and it is a potential important target for multi-gene combined therapeutics.

Keywords

GIPC1 Gene therapy Glioma Neuropilin-1

References

  1. J Biol Chem. 2008 Sep 12;283(37):25110-4 [PMID: 18628209]
  2. J Biomed Nanotechnol. 2013 Apr;9(4):559-63 [PMID: 23621014]
  3. J Clin Oncol. 2008 Oct 1;26(28):4659-65 [PMID: 18824712]
  4. PLoS One. 2012;7(11):e49227 [PMID: 23145131]
  5. FASEB J. 2006 Jul;20(9):1513-5 [PMID: 16754745]
  6. Blood. 2011 Jul 21;118(3):816-26 [PMID: 21586748]
  7. J Biol Chem. 2005 Apr 8;280(14):13457-64 [PMID: 15695515]
  8. Carcinogenesis. 2011 Apr;32(4):613-21 [PMID: 21186301]
  9. World J Biol Chem. 2013 Nov 26;4(4):91-101 [PMID: 24340132]
  10. J Pathol. 2003 Jul;200(3):336-47 [PMID: 12845630]
  11. Oncogene. 2007 Aug 16;26(38):5577-86 [PMID: 17369861]
  12. Proc Natl Acad Sci U S A. 2006 Aug 22;103(34):12735-40 [PMID: 16908842]
  13. Cell. 2013 Feb 28;152(5):1065-76 [PMID: 23452854]
  14. Int J Cancer. 2002 Oct 10;101(5):409-14 [PMID: 12216067]
  15. J Cell Physiol. 2014 Mar;229(3):277-86 [PMID: 23929496]
  16. Clin Cancer Res. 2009 Jun 15;15(12):4095-103 [PMID: 19509165]
  17. Trends Cell Biol. 2006 May;16(5):257-63 [PMID: 16581250]
  18. Mol Cancer Res. 2010 Dec;8(12 ):1591-600 [PMID: 21047775]
  19. Cancer Metastasis Rev. 2013 Jun;32(1-2):147-62 [PMID: 23085856]
  20. Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12340-5 [PMID: 9770488]
  21. Cell Metab. 2012 Jun 6;15(6):827-37 [PMID: 22682223]
  22. Cancer Cell. 2007 Jan;11(1):53-67 [PMID: 17222790]
  23. J Exp Med. 2012 Mar 12;209(3):507-20 [PMID: 22393126]
  24. Int J Cancer. 2000 Mar 20;89(2):167-71 [PMID: 10754495]
  25. Anticancer Res. 2009 Dec;29(12):5171-84 [PMID: 20044633]
  26. Nat Rev Neurosci. 2014 Jul;15(7):455-65 [PMID: 24946761]
  27. Int J Mol Med. 2002 May;9(5):509-13 [PMID: 11956658]
  28. Oncogene. 2001 Oct 11;20(46):6669-78 [PMID: 11709701]
  29. Biochem J. 2011 May 1;435(3):609-18 [PMID: 21306301]
  30. Biochem J. 2008 Apr 15;411(2):211-26 [PMID: 18363553]
  31. Cancer Biol Ther. 2007 Aug;6(8):1173-80 [PMID: 17726369]
  32. Cancer Lett. 2013 May 28;332(2):335-45 [PMID: 21269762]
  33. Anticancer Res. 2004 Mar-Apr;24(2B):547-52 [PMID: 15160992]
  34. Exp Mol Med. 2013 Jun 07;45:e26 [PMID: 23743496]
  35. J Neurosci. 2014 Feb 5;34(6):2169-90 [PMID: 24501358]
  36. Trends Cell Biol. 2004 Nov;14(11):639-47 [PMID: 15519853]
  37. Cell Signal. 2013 Apr;25(4):766-77 [PMID: 23277200]
  38. Cancer Res. 2006 Nov 1;66(21):10264-8 [PMID: 17079444]
  39. Exp Cell Res. 2006 Mar 10;312(5):584-93 [PMID: 16445911]
  40. J Biol Chem. 2003 Dec 5;278(49):48848-60 [PMID: 14514674]
  41. Cancer Lett. 2006 Jan 8;231(1):1-11 [PMID: 16356825]
  42. PLoS Biol. 2009 Jan 27;7(1):e25 [PMID: 19175293]
  43. Cancer Lett. 2010 Aug 28;294(2):254-63 [PMID: 20206441]
  44. EMBO Rep. 2008 Oct;9(10):983-9 [PMID: 18704117]
  45. Cancer Lett. 2015 Jun 28;362(1):1-7 [PMID: 25796440]
  46. Annu Rev Cell Dev Biol. 2007;23:263-92 [PMID: 17539753]
  47. PLoS One. 2007 Nov 14;2(11):e1161 [PMID: 18000534]
  48. Cell. 2004 Feb 6;116(3):445-56 [PMID: 15016378]
  49. J Biomed Nanotechnol. 2013 Apr;9(4):551-8 [PMID: 23621013]
  50. J Neurosci. 1999 Aug 1;19(15):6519-27 [PMID: 10414980]
  51. CA Cancer J Clin. 2010 May-Jun;60(3):166-93 [PMID: 20445000]
  52. BMC Cancer. 2008 Aug 24;8:247 [PMID: 18721484]
  53. Mol Cell Biol. 2006 Dec;26(23):8942-52 [PMID: 17015470]
  54. Am J Pathol. 2004 Jun;164(6):2139-51 [PMID: 15161648]
  55. J Med Chem. 2013 Jul 11;56(13):5219-30 [PMID: 23566315]
  56. Cancer Cell. 2010 Jan 19;17(1):98-110 [PMID: 20129251]
  57. Cell Death Differ. 2009 Jan;16(1):3-11 [PMID: 18846107]
  58. PLoS One. 2014 Jul 07;9(7):e101931 [PMID: 24999732]
  59. FEBS Lett. 2013 Nov 1;587(21):3392-9 [PMID: 24021649]
  60. Biochim Biophys Acta. 2007 Aug;1773(8):1263-84 [PMID: 17126425]

MeSH Term

Adaptor Proteins, Signal Transducing
Animals
Apoptosis
Biomarkers, Tumor
Blotting, Western
Cell Movement
Cell Proliferation
Disease Progression
Flow Cytometry
Fluorescent Antibody Technique
Glioma
Humans
Immunoenzyme Techniques
Immunoprecipitation
Neoplasm Invasiveness
Neuropilin-1
Protein Binding
RNA, Small Interfering
Rats
Signal Transduction
Tumor Cells, Cultured

Chemicals

Adaptor Proteins, Signal Transducing
Biomarkers, Tumor
GIPC1 protein, human
RNA, Small Interfering
Neuropilin-1
Journal Article
Article has an altmetric score of 1

Word Cloud

Created with Highcharts 10.0.0proteingliomaGIPC1NRP-1Neuropilin-1pathwayGliomamulti-geneproliferationinvasioninvolvedsignaling1domaincellsadaptorprogressionoccursdueabnormalitiestransmembraneinvolvesmigrationwelltumorangiogenesiscytoplasmicGAIP/RGS19-interactingregulateclathrin-vesiclestraffickingrecyclingshowco-localizesco-immunoprecipitatesC-terminalSEA-COOHmotifinteractsspeciallynamedthreeproteins:PSD-9595kDapost-synapticdensityDLGDrosophilamelanogasterDiscsLargeZO-1zonulaoccludensmaintenanceepithelialpolarityPDZKnockdownsmallinterferingRNAsiRNAsignificantlyreducesvitroincreasesapoptosisFurthermoresi-GIPC1preventsactionproteinsphosphotyrosineinteractionPHleucinezippercontainingAPPL1p130CasinhibitsdownstreamkirstenratsarcomaviraloncogenehomologKRAS-ERKstudydemonstratedNRP-1/GIPC1playsvitalrolepotentialimportanttargetcombinedtherapeutics/GIPC1mediatesGenetherapy

Similar Articles

Cited By (19)