- T D Dreesen: Department of Biochemistry, Drawer BB, 39762, Mississippi State, MS.
- M Dickens: Department of Biochemistry, Drawer BB, 39762, Mississippi State, MS.
- R B Koch: Department of Biochemistry, Drawer BB, 39762, Mississippi State, MS.
A highly purified Ca(2+)-stimulated lipoxygenase was isolated from the Hill variety of soybean seeds. Separation of Ca(2+)-stimulated lipoxygenase from lipoxygenase active in the absence of Ca(2+) (lipoxygenase-1) was readily obtained using a DEAE-cellulose column. Sample size applied to the ion exchange column was found to be critical. Both enzymes were bound to the column, although some highly active Ca(2+)-stimulated lipoxygenase eluted with buffer in the presence of bound lipoxygenase-1. Ca(2+)-stimulated lipoxygenase bound to DAAE-cellulose required the use of a NaCl gradient for elution. Ca(2+)-stimulated lipoxygenase showed an apparent isoelectric point at pH 5.90 and optimum activity at pH 7.5 and at 1.1 mM calcium. Lipoxygenase-1 was inhibited over 95% in the presence of 60 μM methyl mercuric chloride, while Ca(2+)-stimulated lipoxygenase showed a maximum of only 20% inhibition under the same conditions.