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Journal of immunology research
2016;2016 7801781.

Bone Marrow Mesenchymal Stem Cells Enhance the Differentiation of Human Switched Memory B Lymphocytes into Plasma Cells in Serum-Free Medium.

Guillaume Bonnaure, Catherine Gervais-St-Amour, Sonia Néron
Author Information
  1. Guillaume Bonnaure: Hema-Quebec's Department of Research and Development, 1070 Avenue des Sciences-de-la-Vie, Québec, QC, Canada G1V 5C3; Department of Biochemistry, Microbiology and Bioinformatics, Laval University, 1045 Avenue de la Médecine, Québec, QC, Canada G1V 0A6. ORCID
  2. Catherine Gervais-St-Amour: Hema-Quebec's Department of Research and Development, 1070 Avenue des Sciences-de-la-Vie, Québec, QC, Canada G1V 5C3; Department of Biochemistry, Microbiology and Bioinformatics, Laval University, 1045 Avenue de la Médecine, Québec, QC, Canada G1V 0A6.
  3. Sonia Néron: Hema-Quebec's Department of Research and Development, 1070 Avenue des Sciences-de-la-Vie, Québec, QC, Canada G1V 5C3; Department of Biochemistry, Microbiology and Bioinformatics, Laval University, 1045 Avenue de la Médecine, Québec, QC, Canada G1V 0A6. ORCID
PMID: 27872867 DOI: 10.1155/2016/7801781

Abstract

The differentiation of human B lymphocytes into plasma cells is one of the most stirring questions with regard to adaptive immunity. However, the terminal differentiation and survival of plasma cells are still topics with much to be discovered, especially when targeting switched memory B lymphocytes. Plasma cells can migrate to the bone marrow in response to a CXCL12 gradient and survive for several years while secreting antibodies. In this study, we aimed to get closer to niches favoring plasma cell survival. We tested low oxygen concentrations and coculture with mesenchymal stem cells (MSC) from human bone marrow. Besides, all cultures were performed using an animal protein-free medium. Overall, our model enables the generation of high proportions of CD38CD138CD31 plasma cells (≥50%) when CD40-activated switched memory B lymphocytes were cultured in direct contact with mesenchymal stem cells. In these cultures, the secretion of CXCL12 and TGF-, usually found in the bone marrow, was linked to the presence of MSC. The level of oxygen appeared less impactful than the contact with MSC. This study shows for the first time that expanded switched memory B lymphocytes can be differentiated into plasma cells using exclusively a serum-free medium.

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MeSH Term

B-Lymphocytes
Biomarkers
CD40 Antigens
Cell Differentiation
Cells, Cultured
Culture Media, Serum-Free
Cytokines
Humans
Immunologic Memory
Immunophenotyping
Lymphocyte Activation
Mesenchymal Stem Cells
Oxidation-Reduction
Oxygen
Plasma Cells

Chemicals

Biomarkers
CD40 Antigens
Culture Media, Serum-Free
Cytokines
Oxygen
Journal Article

Word Cloud

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