Isolation and Characterization of a Bacteriophage Preying an Antifungal Bacterium.

Aryan Rahimi-Midani, Kyoung-Ho Kim, Seon-Woo Lee, Sang Bong Jung, Tae-Jin Choi
Author Information
  1. Aryan Rahimi-Midani: Department of Microbiology, Pukyong National University, Busan 48547, Korea.
  2. Kyoung-Ho Kim: Department of Microbiology, Pukyong National University, Busan 48547, Korea.
  3. Seon-Woo Lee: Department of Applied Biology, Dong-A University, Busan 49315, Korea.
  4. Sang Bong Jung: Department of Clinical Laboratory Science, Dong-Eui Institute of Technology, Busan 47230, Korea.
  5. Tae-Jin Choi: Department of Microbiology, Pukyong National University, Busan 48547, Korea.

Abstract

Several species were isolated from rice field soils, and 16S rRNA gene sequence analysis showed that was the most abundant. A strain named BC1 showed antifungal activity against . Bacteriophages infecting strain BC1 were isolated from the same soil sample. The isolated phage PK16 had an icosahedral head of 100 ± 5 nm and tail of 200 ± 5 nm, indicating that it belonged to the family . Analysis of the complete linear dsDNA genome revealed a 158,127-bp genome with G + C content of 39.9% comprising 235 open reading frames as well as 19 tRNA genes (including 1 pseudogene). Blastp analysis showed that the proteins encoded by the PK16 genome had the closest hits to proteins of seven different bacteriophages. A neighbor-joining phylogenetic tree based on the major capsid protein showed a robust clustering of phage PK16 with phage JBP901 and BCP8-2 isolated from Korean fermented food.

Keywords

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Word Cloud

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