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Plastic and reconstructive surgery. Global open
Nov, 2016;4 (11): e1073.

Effect of Previous Irradiation on Vascular Thrombosis of Microsurgical Anastomosis: A Preclinical Study in Rats.

Sergi Barrera-Ochoa, Irene Gallardo-Calero, Alba López-Fernández, Cleofe Romagosa, Ramona Vergés, Marius Aguirre-Canyadell, Francisco Soldado, Roberto Velez
Author Information
  1. Sergi Barrera-Ochoa: Orthopedic Surgery Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; Hand and Microsurgery Unit, ICATME, Hospital Universitari Quiron-Dexeus-Grupo Quirónsalud, Barcelona, Spain; Musculoskeletal Tissue Engineering Group, Vall d'Hebron Research Institute (VHIR), Barcelona, Spain; Pathology Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; Radiotherapy Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; and Pediatric Upper Extremity Surgery and Microsurgery Department, Hospital Universitari Sant Joan de Déu, Barcelona, Spain.
  2. Irene Gallardo-Calero: Orthopedic Surgery Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; Hand and Microsurgery Unit, ICATME, Hospital Universitari Quiron-Dexeus-Grupo Quirónsalud, Barcelona, Spain; Musculoskeletal Tissue Engineering Group, Vall d'Hebron Research Institute (VHIR), Barcelona, Spain; Pathology Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; Radiotherapy Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; and Pediatric Upper Extremity Surgery and Microsurgery Department, Hospital Universitari Sant Joan de Déu, Barcelona, Spain.
  3. Alba López-Fernández: Orthopedic Surgery Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; Hand and Microsurgery Unit, ICATME, Hospital Universitari Quiron-Dexeus-Grupo Quirónsalud, Barcelona, Spain; Musculoskeletal Tissue Engineering Group, Vall d'Hebron Research Institute (VHIR), Barcelona, Spain; Pathology Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; Radiotherapy Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; and Pediatric Upper Extremity Surgery and Microsurgery Department, Hospital Universitari Sant Joan de Déu, Barcelona, Spain.
  4. Cleofe Romagosa: Orthopedic Surgery Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; Hand and Microsurgery Unit, ICATME, Hospital Universitari Quiron-Dexeus-Grupo Quirónsalud, Barcelona, Spain; Musculoskeletal Tissue Engineering Group, Vall d'Hebron Research Institute (VHIR), Barcelona, Spain; Pathology Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; Radiotherapy Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; and Pediatric Upper Extremity Surgery and Microsurgery Department, Hospital Universitari Sant Joan de Déu, Barcelona, Spain.
  5. Ramona Vergés: Orthopedic Surgery Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; Hand and Microsurgery Unit, ICATME, Hospital Universitari Quiron-Dexeus-Grupo Quirónsalud, Barcelona, Spain; Musculoskeletal Tissue Engineering Group, Vall d'Hebron Research Institute (VHIR), Barcelona, Spain; Pathology Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; Radiotherapy Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; and Pediatric Upper Extremity Surgery and Microsurgery Department, Hospital Universitari Sant Joan de Déu, Barcelona, Spain.
  6. Marius Aguirre-Canyadell: Orthopedic Surgery Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; Hand and Microsurgery Unit, ICATME, Hospital Universitari Quiron-Dexeus-Grupo Quirónsalud, Barcelona, Spain; Musculoskeletal Tissue Engineering Group, Vall d'Hebron Research Institute (VHIR), Barcelona, Spain; Pathology Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; Radiotherapy Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; and Pediatric Upper Extremity Surgery and Microsurgery Department, Hospital Universitari Sant Joan de Déu, Barcelona, Spain.
  7. Francisco Soldado: Orthopedic Surgery Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; Hand and Microsurgery Unit, ICATME, Hospital Universitari Quiron-Dexeus-Grupo Quirónsalud, Barcelona, Spain; Musculoskeletal Tissue Engineering Group, Vall d'Hebron Research Institute (VHIR), Barcelona, Spain; Pathology Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; Radiotherapy Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; and Pediatric Upper Extremity Surgery and Microsurgery Department, Hospital Universitari Sant Joan de Déu, Barcelona, Spain.
  8. Roberto Velez: Orthopedic Surgery Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; Hand and Microsurgery Unit, ICATME, Hospital Universitari Quiron-Dexeus-Grupo Quirónsalud, Barcelona, Spain; Musculoskeletal Tissue Engineering Group, Vall d'Hebron Research Institute (VHIR), Barcelona, Spain; Pathology Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; Radiotherapy Department, Hospital Universitari Vall d'Hebron, Barcelona, Spain; and Pediatric Upper Extremity Surgery and Microsurgery Department, Hospital Universitari Sant Joan de Déu, Barcelona, Spain.
PMID: 27975009 DOI: 10.1097/GOX.0000000000001073

Abstract

BACKGROUND: The objective of the present investigation was to compare the effect of neoadjuvant irradiation on the microvascular anastomosis in cervical bundle using an experimental model in rats.
METHODS: One hundred forty male Sprague-Dawley rats were allocated into 4 groups: group I, control, arterial microanastomosis; group II, control, venous microanastomosis; group III, arterial microanastomosis with previous irradiation (20 Gy); and group IV, venous microanastomosis with previous irradiation (20 Gy). Clinical parameters, technical values of anastomosis, patency, and histopathological parameters were evaluated.
RESULTS: Irradiated groups (III and IV) and vein anastomosis groups (II and IV) showed significantly increased technical difficulties. Group IV showed significantly reduced patency rates (7/35) when compared with the control group (0/35). Radiotherapy significantly decreased the patency rates of the vein (7/35) when compared with the artery (1/35). Groups III and IV showed significantly reduced number of endothelial cells and also showed the presence of intimal thickening and adventitial fibrosis as compared with the control group.
CONCLUSION: Neoadjuvant radiotherapy reduces the viability of the venous anastomosis in a preclinical rat model with a significant increase in the incidence of vein thrombosis.

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Created with Highcharts 10.0.0groupIVanastomosiscontrolmicroanastomosisshowedsignificantlyirradiationvenousIIIpatencyveincomparedmodelratsarterialIIprevious20Gyparameterstechnicalgroupsreducedrates7/35BACKGROUND:objectivepresentinvestigationcompareeffectneoadjuvantmicrovascularcervicalbundleusingexperimentalMETHODS:OnehundredfortymaleSprague-Dawleyallocated4groups:ClinicalvalueshistopathologicalevaluatedRESULTS:IrradiatedincreaseddifficultiesGroup0/35Radiotherapydecreasedartery1/35GroupsnumberendothelialcellsalsopresenceintimalthickeningadventitialfibrosisCONCLUSION:NeoadjuvantradiotherapyreducesviabilitypreclinicalratsignificantincreaseincidencethrombosisEffectPreviousIrradiationVascularThrombosisMicrosurgicalAnastomosis:PreclinicalStudyRats

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