Force Measurements for Cancer Cells.

Advanced Search

Vivek Rajasekharan, Varun K A Sreenivasan, Brenda Farrell

Author Information

Vivek Rajasekharan: Bobby R. Alford Department of Otolaryngology - Head & Neck Surgery, Baylor College of Medicine, Houston, TX, 77030, USA.
Varun K A Sreenivasan: Bobby R. Alford Department of Otolaryngology - Head & Neck Surgery, Baylor College of Medicine, Houston, TX, 77030, USA.
Brenda Farrell: Bobby R. Alford Department of Otolaryngology - Head & Neck Surgery, Baylor College of Medicine, Houston, TX, 77030, USA. bfarrell@bcm.edu.

PMID: 28150204 DOI: 10.1007/978-1-4939-6646-2_12

During cytoskeleton remodeling, cancer cells generate force at the plasma membrane that originates from chemical motors (e.g., actin). This force (pN) and its time course reflect the on and off-rates of the motors. We describe the design and calibration of a force-measuring device (i.e., optical tweezers) that is used to monitor this force and its time course at the edge of a cell, with particular emphasis on the temporal resolution of the instrument.

Actin Cell mobility Cytoskeleton Filopodium Membrane force Membrane tube Motor kinetics Noise of signal Optical tweezers Time constant

R21 CA152779/NCI NIH HHS
S10 RR027549/NCRR NIH HHS

Algorithms

Biomechanical Phenomena

Cell Movement

Models, Theoretical

Optical Tweezers

Optics and Photonics

Signal-To-Noise Ratio

Temperature

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

OpenLB
Open Library of Bioscience