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03 27, 2017;7 (1): 439.

Transcriptome and functional analysis reveals hybrid vigor for oil biosynthesis in oil palm.

Jingjing Jin, Yanwei Sun, Jing Qu, Rahmad Syah, Chin-Huat Lim, Yuzer Alfiko, Nur Estya Bte Rahman, Antonius Suwanto, Genhua Yue, Limsoon Wong, Nam-Hai Chua, Jian Ye
Author Information
  1. Jingjing Jin: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604, NUS, Singapore.
  2. Yanwei Sun: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604, NUS, Singapore.
  3. Jing Qu: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604, NUS, Singapore.
  4. Rahmad Syah: R&D Department, Wilmar International Plantation, Palembang, Indonesia Biotech Lab, Wilmar International, Jakarta, Indonesia.
  5. Chin-Huat Lim: R&D Department, Wilmar International Plantation, Palembang, Indonesia Biotech Lab, Wilmar International, Jakarta, Indonesia.
  6. Yuzer Alfiko: R&D Department, Wilmar International Plantation, Palembang, Indonesia Biotech Lab, Wilmar International, Jakarta, Indonesia.
  7. Nur Estya Bte Rahman: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604, NUS, Singapore.
  8. Antonius Suwanto: R&D Department, Wilmar International Plantation, Palembang, Indonesia Biotech Lab, Wilmar International, Jakarta, Indonesia.
  9. Genhua Yue: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604, NUS, Singapore.
  10. Limsoon Wong: School of Computing, National University of Singapore, 117417, NUS, Singapore. ORCID
  11. Nam-Hai Chua: Laboratory of Plant Molecular Biology, Rockefeller University, 1230 York Avenue, New York, NY, 10021, USA.
  12. Jian Ye: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604, NUS, Singapore. jianye@im.ac.cn. ORCID
PMID: 28348403 DOI: 10.1038/s41598-017-00438-8

Abstract

Oil palm is the most productive oil crop in the world and composes 36% of the world production. However, the molecular mechanisms of hybrids vigor (or heterosis) between Dura, Pisifera and their hybrid progeny Tenera has not yet been well understood. Here we compared the temporal and spatial compositions of lipids and transcriptomes for two oil yielding organs mesocarp and endosperm from Dura, Pisifera and Tenera. Multiple lipid biosynthesis pathways are highly enriched in all non-additive expression pattern in endosperm, while cytokinine biosynthesis and cell cycle pathways are highly enriched both in endosperm and mesocarp. Compared with parental palms, the high oil content in Tenera was associated with much higher transcript levels of EgWRI1, homolog of Arabidopsis thaliana WRINKLED1. Among 338 identified genes in lipid synthesis, 207 (61%) has been identified to contain the WRI1 specific binding AW motif. We further functionally identified EgWRI1-1, one of three EgWRI1 orthologs, by genetic complementation of the Arabidopsis wri1 mutant. Ectopic expression of EgWRI1-1 in plant produced dramatically increased seed mass and oil content, with oil profile changed. Our findings provide an explanation for EgWRI1 as an important gene contributing hybrid vigor in lipid biosynthesis in oil palm.

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MeSH Term

Arabidopsis
Arabidopsis Proteins
Arecaceae
Biosynthetic Pathways
Chimera
Gene Expression Profiling
Genetic Complementation Test
Hybrid Vigor
Lipids
Palm Oil
Transcription Factors

Chemicals

Arabidopsis Proteins
Lipids
Transcription Factors
WRINKLED1 protein, Arabidopsis
Palm Oil
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 1

Word Cloud

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