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04 21, 2017;7 (1): 1048.

Differential sensitivity to infections and antimicrobial peptide-mediated immune response in four silkworm strains with different geographical origin.

Ottavia Romoli, Alessio Saviane, Andrea Bozzato, Paola D'Antona, Gianluca Tettamanti, Andrea Squartini, Silvia Cappellozza, Federica Sandrelli
Author Information
  1. Ottavia Romoli: Department of Biology, University of Padova, Padova, Italy.
  2. Alessio Saviane: CREA - Honey Bee and Silkworm Research Unit, Padova Seat, Padova, Italy.
  3. Andrea Bozzato: Department of Biology, University of Padova, Padova, Italy.
  4. Paola D'Antona: Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.
  5. Gianluca Tettamanti: Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy. ORCID
  6. Andrea Squartini: Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Padova, Italy.
  7. Silvia Cappellozza: CREA - Honey Bee and Silkworm Research Unit, Padova Seat, Padova, Italy.
  8. Federica Sandrelli: Department of Biology, University of Padova, Padova, Italy. federica.sandrelli@unipd.it. ORCID
PMID: 28432358 DOI: 10.1038/s41598-017-01162-z

Abstract

The domesticated silkworm Bombyx mori has an innate immune system, whose main effectors are the antimicrobial peptides (AMPs). Silkworm strains are commonly grouped into four geographical types (Japanese, Chinese, European and Tropical) and are generally characterised by a variable susceptibility to infections. To clarify the genetic and molecular mechanisms on which the different responses to infections are based, we exposed one silkworm strain for each geographical area to oral infections with the silkworm pathogens Enterococcus mundtii or Serratia marcescens. We detected a differential susceptibility to both bacteria, with the European strain displaying the lowest sensitivity to E. mundtii and the Indian one to S. marcescens. We found that all the strains were able to activate the AMP response against E. mundtii. However, the highest tolerance of the European strain appeared to be related to the specific composition of its AMP cocktail, containing more effective variants such as a peculiar Cecropin B6 isoform. The resistance of the Indian strain to S. marcescens seemed to be associated with its prompt capability to activate the systemic transcription of AMPs. These data suggest that B. mori strains with distinct genetic backgrounds employ different strategies to counteract bacterial infections, whose efficacy appears to be pathogen-dependent.

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MeSH Term

Animals
Anti-Infective Agents
Antimicrobial Cationic Peptides
Bacterial Infections
Bombyx
Disease Susceptibility
Enterococcus
Serratia marcescens

Chemicals

Anti-Infective Agents
Antimicrobial Cationic Peptides
Comparative Study Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 2

Word Cloud

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