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06 14, 2017;7 (1): 3468.

Differential effects of a post-anthesis heat stress on wheat (Triticum aestivum L.) grain proteome determined by iTRAQ.

Yufeng Zhang, Jiajia Pan, Xiuwen Huang, Dandan Guo, Hongyao Lou, Zhenghong Hou, Meng Su, Rongqi Liang, Chaojie Xie, Mingshan You, Baoyun Li
Author Information
  1. Yufeng Zhang: Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy, China Agricultural University, Beijing, 100193, China.
  2. Jiajia Pan: Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy, China Agricultural University, Beijing, 100193, China.
  3. Xiuwen Huang: Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy, China Agricultural University, Beijing, 100193, China.
  4. Dandan Guo: Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy, China Agricultural University, Beijing, 100193, China.
  5. Hongyao Lou: Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy, China Agricultural University, Beijing, 100193, China.
  6. Zhenghong Hou: Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy, China Agricultural University, Beijing, 100193, China.
  7. Meng Su: Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy, China Agricultural University, Beijing, 100193, China.
  8. Rongqi Liang: Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy, China Agricultural University, Beijing, 100193, China.
  9. Chaojie Xie: Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy, China Agricultural University, Beijing, 100193, China.
  10. Mingshan You: Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy, China Agricultural University, Beijing, 100193, China.
  11. Baoyun Li: Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis & Utilization, Ministry of Education, College of Agronomy, China Agricultural University, Beijing, 100193, China. baoyunli@cau.edu.cn.
PMID: 28615669 DOI: 10.1038/s41598-017-03860-0

Abstract

Heat stress, a major abiotic stressor of wheat (Triticum aestivum L.), often results in reduced yield and decreased quality. In this study, a proteomic method, Tags for Relative and Absolute Quantitation Isobaric (iTRAQ), was adopted to analyze the protein expression profile changes among wheat cultivar Jing411 under heat stress. Results indicated that there were 256 different proteins expressed in Jing411 under heat stress. According to the result of gene annotation and functional classification, 239 proteins were annotated by 856 GO function entries, including growth and metabolism proteins, energy metabolism proteins, processing and storage proteins, defense-related proteins, signal transduction, unknown function proteins and hypothetical proteins. GO enrichment analysis suggested that the differentially expressed proteins in Jing411 under heat stress were mainly involved in stimulus response (67), abiotic stress response (26) and stress response (58), kinase activity (12), and transferase activity (12). Among the differentially expressed proteins in Jing411, 115 were attributed to 119 KEGG signaling/metabolic pathways. KEGG pathway enrichment analysis in Jing411 showed that heat stress mainly affected the starch and sucrose metabolism as well as protein synthesis pathway in the endoplasmic reticulum. The protein interaction network indicated that there were 8 differentially expressed proteins that could form an interaction network in Jing411.

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MeSH Term

Computational Biology
Gene Ontology
Heat-Shock Response
Hot Temperature
Molecular Sequence Annotation
Plant Proteins
Protein Interaction Mapping
Protein Interaction Maps
Proteome
Proteomics
Triticum

Chemicals

Plant Proteins
Proteome
Journal Article Research Support, Non-U.S. Gov't
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Word Cloud

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