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09 21, 2017;7 (1): 12087.

Overexpression of annexin gene AnnSp2, enhances drought and salt tolerance through modulation of ABA synthesis and scavenging ROS in tomato.

Raina Ijaz, Javeria Ejaz, Shenghua Gao, Tengfei Liu, Muhammad Imtiaz, Zhibiao Ye, Taotao Wang
Author Information
  1. Raina Ijaz: Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.
  2. Javeria Ejaz: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
  3. Shenghua Gao: Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.
  4. Tengfei Liu: Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.
  5. Muhammad Imtiaz: Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, China.
  6. Zhibiao Ye: Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.
  7. Taotao Wang: Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China. ttwang@mail.hzau.edu.cn.
PMID: 28935951 DOI: 10.1038/s41598-017-11168-2

Abstract

Drought and high salinity are two major abiotic stresses that significantly affect agricultural crop productivity worldwide. Annexins are a multigene family that plays an essential role in plant stress responses and various cellular processes. Here, the AnnSp2 gene was cloned from drought-resistant wild tomato (Solanum pennellii) and functionally characterized in cultivated tomato. AnnSp2 protein was localized in the nucleus and had higher expression in leave, flower and fruit. It was induced by several phytohormones and some abiotic stresses. Tomato plants overexpressing AnnSp2 had increased tolerance to drought and salt stress, as determined by analysis of various physiological parameters. AnnSp2-transgenic plants were less sensitive to ABA during the seed germination and seedling stages. However, under drought stress, the ABA content significantly increased in the AnnSp2-overexpressing plants, inducing stomatal closure and reducing water loss, which underlay the plants' enhanced stress tolerance. Furthermore, scavenging reactive oxygen species (ROS), higher total chlorophyll content, lower lipid peroxidation levels, increased peroxidase activities (including APX, CAT and SOD) and higher levels of proline were observed in AnnSp2-overexpressing plants. These results indicate that overexpression of AnnSp2 in transgenic tomato improves salt and drought tolerance through ABA synthesis and the elimination of ROS.

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MeSH Term

Abscisic Acid
Amino Acid Sequence
Annexins
Droughts
Gene Expression Profiling
Gene Expression Regulation, Plant
Peroxidase
Phylogeny
Plant Growth Regulators
Plant Proteins
Plants, Genetically Modified
Reactive Oxygen Species
Salt Tolerance
Sequence Homology, Amino Acid
Solanum

Chemicals

Annexins
Plant Growth Regulators
Plant Proteins
Reactive Oxygen Species
Abscisic Acid
Peroxidase
Journal Article Research Support, Non-U.S. Gov't

Word Cloud

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