Multilaboratory Evaluation of a Novel Lateral Flow Immunochromatographic Assay for Confirming Isolation of Mycobacterium bovis from Veterinary Diagnostic Specimens.
Linda D Stewart, Lyanne McCallan, James McNair, Adrian McGoldrick, Rowan Morris, Jean-Louis Moyen, Lucía De Juan Ferré, Beatriz Romero, Elena Alonso, Sven D C Parsons, Paul Van Helden, Flábio R Araújo, Irene R Grant
Author Information
Linda D Stewart: Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast, Northern Ireland, United Kingdom.
Lyanne McCallan: Veterinary Sciences Division, Agri-Food and Biosciences Institute, Belfast, Northern Ireland, United Kingdom.
James McNair: Veterinary Sciences Division, Agri-Food and Biosciences Institute, Belfast, Northern Ireland, United Kingdom.
Adrian McGoldrick: Animal and Plant Health Agency Veterinary Inspection Centre Starcross, Exeter, United Kingdom.
Rowan Morris: Animal and Plant Health Agency Veterinary Inspection Centre Starcross, Exeter, United Kingdom.
Jean-Louis Moyen: Laboratoire Départemental d'Analyse et de Recherche de la Dordogne, Coulounieix-Chamiers, France.
Lucía De Juan Ferré: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Madrid, Spain.
Beatriz Romero: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Madrid, Spain.
Elena Alonso: Laboratorio Regional de Sanidad Animal de la Junta de Castilla y León, León, Spain.
Sven D C Parsons: DST/NRF Centre of Excellence for Biomedical Tuberculosis Research/SAMRC Centre for TB Research/Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa. ORCID
Paul Van Helden: DST/NRF Centre of Excellence for Biomedical Tuberculosis Research/SAMRC Centre for TB Research/Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa.
Flábio R Araújo: Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA Gado de Corte), Campo Grande, Brazil.
Irene R Grant: Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast, Northern Ireland, United Kingdom i.grant@qub.ac.uk. ORCID
A novel lateral flow immunochromatographic device (LFD) was evaluated in several veterinary diagnostic laboratories. It was confirmed to be specific for and cells. The performance of the novel LFD was assessed relative to the confirmatory tests routinely applied after culture (spoligotyping or quantitative PCR [qPCR]) in each laboratory; liquid (MGIT or BacT/Alert) and/or solid (Stonebrink, Coletsos, or Lowenstein-Jensen) cultures were tested. In comparison to spoligotyping of acid-fast-positive MGIT cultures, percent agreement between positive LFD and spoligotyping results was excellent in two United Kingdom laboratories (97.7 to 100%) but lower in the Spanish context (76%), where spoligotyping was applied to MGIT cultures previously confirmed to be positive for complex (MTBC) by qPCR. Certain spoligotypes of and were not detected by the LFD in Spanish MGIT cultures. Compared to qPCR confirmation, the agreement between positive LFD and qPCR results was 42.3% and 50% for BacT/Alert and MGIT liquid cultures, respectively, and for solid cultures, it ranged from 11.1 to 89.2%, depending on the solid medium employed (Coletsos, 11.1%; Lowenstein-Jensen, 55.6%; Stonebrinks, 89.2%). Correlation between the novel LFD and BD MGIT TBc Identification test results was excellent when 190 MGIT cultures were tested ( = 0.9791; < 0.0001), with the added benefit that was differentiated from another MTBC species in one MGIT culture by the novel LFD. This multilaboratory evaluation demonstrated the novel LFD's potential utility as a rapid test to confirm isolation of and from veterinary specimens following culture.
