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PloS one
2017;12 (10): e0186415.

A novel bacteriocin from Enterococcus faecalis 478 exhibits a potent activity against vancomycin-resistant enterococci.

Uraporn Phumisantiphong, Kanokrat Siripanichgon, Onrapak Reamtong, Pornphan Diraphat
Author Information
  1. Uraporn Phumisantiphong: Department of Microbiology, Faculty of Public Health, Mahidol University, Bangkok, Thailand.
  2. Kanokrat Siripanichgon: Department of Microbiology, Faculty of Public Health, Mahidol University, Bangkok, Thailand.
  3. Onrapak Reamtong: Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
  4. Pornphan Diraphat: Department of Microbiology, Faculty of Public Health, Mahidol University, Bangkok, Thailand. ORCID
PMID: 29023515 DOI: 10.1371/journal.pone.0186415

Abstract

The emergence of multidrug-resistant enterococci (MDRE) and particularly vancomycin-resistant enterococci (VRE) is considered a serious health problem worldwide, causing the need for new antimicrobials. The aim of this study was to discover and characterize bacteriocin against clinical isolates of MDRE and VRE. Over 10,000 bacterial isolates from water, environment and clinical samples were screened. E. faecalis strain 478 isolated from human feces produced the highest antibacterial activity against several MDRE and VRE strains. The optimum condition for bacteriocin production was cultivation in MRS broth at 37°C, pH 5-6 for 16 hours. The bacteriocin-like substance produced from E. faecalis strain EF478 was stable at 60°C for at least 1 hour and retained its antimicrobial activity after storage at -20°C for 1 year, at 4°C for 6 months, and at 25°C for 2 months. A nano-HPLC electrospray ionization multi-stage tandem mass spectrometry (nLC-ESI-MS/MS) analysis showed that the amino acid sequences of the bacteriocin-like substance was similar to serine protease of E. faecalis, gi|488296663 (NCBI database), which has never been reported as a bacteriocin. This study reported a novel bacteriocin with high antibacterial activity against VRE and MDRE.

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MeSH Term

Amino Acid Sequence
Anti-Infective Agents
Bacteriocins
DNA
Enterococcus faecalis
Feces
Humans
Hydrogen-Ion Concentration
Molecular Sequence Data
Nanotechnology
Protein Structure, Tertiary
Sequence Alignment
Sequence Analysis, DNA
Serine Proteases
Spectrometry, Mass, Electrospray Ionization
Temperature
Vancomycin-Resistant Enterococci

Chemicals

Anti-Infective Agents
Bacteriocins
DNA
Serine Proteases
Journal Article
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