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Veterinary medicine and science
Aug, 2016;2 (3): 154-160.

Molecular prevalence of DNA in goats' milk and seroprevalence in Northwest Tunisia.

Safa Amairia, Mariem Rouatbi, Mohamed R Rjeibi, Hanen Nouasri, Limam Sassi, Moez Mhadhbi, Mohamed Gharbi
Author Information
  1. Safa Amairia: Laboratoire de ParasitologieUniv. ManoubaInstitution de la Recherche et de l'Enseignement Supérieur AgricolesÉcole Nationale de Médecine Vétérinaire de Sidi ThabetSidi Thabet2020Tunisia.
  2. Mariem Rouatbi: Laboratoire de ParasitologieUniv. ManoubaInstitution de la Recherche et de l'Enseignement Supérieur AgricolesÉcole Nationale de Médecine Vétérinaire de Sidi ThabetSidi Thabet2020Tunisia.
  3. Mohamed R Rjeibi: Laboratoire de ParasitologieUniv. ManoubaInstitution de la Recherche et de l'Enseignement Supérieur AgricolesÉcole Nationale de Médecine Vétérinaire de Sidi ThabetSidi Thabet2020Tunisia.
  4. Hanen Nouasri: Laboratoire de ParasitologieUniv. ManoubaInstitution de la Recherche et de l'Enseignement Supérieur AgricolesÉcole Nationale de Médecine Vétérinaire de Sidi ThabetSidi Thabet2020Tunisia.
  5. Limam Sassi: Laboratoire de ParasitologieUniv. ManoubaInstitution de la Recherche et de l'Enseignement Supérieur AgricolesÉcole Nationale de Médecine Vétérinaire de Sidi ThabetSidi Thabet2020Tunisia.
  6. Moez Mhadhbi: Laboratoire de ParasitologieUniv. ManoubaInstitution de la Recherche et de l'Enseignement Supérieur AgricolesÉcole Nationale de Médecine Vétérinaire de Sidi ThabetSidi Thabet2020Tunisia.
  7. Mohamed Gharbi: Laboratoire de ParasitologieUniv. ManoubaInstitution de la Recherche et de l'Enseignement Supérieur AgricolesÉcole Nationale de Médecine Vétérinaire de Sidi ThabetSidi Thabet2020Tunisia.
PMID: 29067190 DOI: 10.1002/vms3.29

Abstract

Toxoplasmosis is a worldwide zoonosis with high impact on human and animal health. Consumption of unpasteurized milk is a risk factor of human toxoplasmosis. The aim of this study was to estimate the seroprevalence and molecular prevalence of in goats' milk in Northwest of Tunisia (Jendouba Governorate). A total number of 77 blood samples were collected from six herds were screened with a commercial ELISA kit for antibodies. For the same goats' samples, a nested PCR was performed to detect DNA in milk. The seroprevalence of infection was 31.2% (±0.05) while the molecular prevalence of this parasite in milk was estimated to 7.8% (±0.03). A very low value of kappa showed that there is not agreement between seroprevalence and parasite prevalence in milk. These results suggest that the consumption of raw milk from naturally infected goats is a potential source of human infection. An extension programme should be implemented to decrease related to goats' raw milk consumption.

Keywords

ELISA PCR Toxoplasma gondii Tunisia goat milk

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Journal Article
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Word Cloud

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