Jiafang Fu: Shandong Medicinal Biotechnology Center, Shandong Academy of Medical Sciences, Jinan, Jingshi Road 18877, Jinan 250062, Shandong, China.
Gongli Zong: Shandong Medicinal Biotechnology Center, Shandong Academy of Medical Sciences, Jinan, Jingshi Road 18877, Jinan 250062, Shandong, China.
Peipei Zhang: State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nanlu, Jinan 250100, China.
Zhilong Zhao: School of Phaemacy, Linyi University, Linyi, China.
Junxia Ma: Shandong Medicinal Biotechnology Center, Shandong Academy of Medical Sciences, Jinan, Jingshi Road 18877, Jinan 250062, Shandong, China.
Xiuhua Pang: State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nanlu, Jinan 250100, China.
Guangxiang Cao: Shandong Medicinal Biotechnology Center, Shandong Academy of Medical Sciences, Jinan, Jingshi Road 18877, Jinan 250062, Shandong, China.
The xdhR gene encodes a TetR-family regulator in Streptomyces coelicolor. However, little is known about the function of XdhR in regulating actinorhodin production. Here, we report that XdhR negatively regulates actinorhodin biosynthesis in S. coelicolor. Deletion of xdhR resulted in overproduction of actinorhodin by approximately 2.5-fold compared to the wild-type strain. Complementation of the xdhR deletion strain restored actinorhodin production to normal levels. In addition, the relative expression levels of actinorhodin cluster genes were all significantly increased in the xdhR deletion strain compared to the wild-type strain. XdhR can specifically bind the promoters of actII-4 and actII-1, two pathway-specific regulators of actinorhodin biosynthesis. These results suggest that xdhR negatively controls actinorhodin biosynthesis by directly regulating actII-4 and actII-1 in S. coelicolor.
