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Nov 10, 2017;7 (1): 199.

Characterization of a novel yeast species Metschnikowia persimmonesis KCTC 12991BP (KIOM G15050 type strain) isolated from a medicinal plant, Korean persimmon calyx (Diospyros kaki Thumb).

Young Min Kang, Ji Eun Choi, Richard Komakech, Jeong Hwan Park, Dae Wook Kim, Kye Man Cho, Seung Mi Kang, Sang Haeng Choi, Kun Chul Song, Chung Min Ryu, Keun Chul Lee, Jung-Sook Lee
Author Information
  1. Young Min Kang: Korea Institute of Oriental Medicine (KIOM), 1672 Yuseong-daero, Yuseong-gu, Daejeon, 34054, Republic of Korea. ymkang@kiom.re.kr. ORCID
  2. Ji Eun Choi: Korea Institute of Oriental Medicine (KIOM), 1672 Yuseong-daero, Yuseong-gu, Daejeon, 34054, Republic of Korea.
  3. Richard Komakech: University of Science & Technology (UST), Korean Medicine Life Science, Campus of KIOM, 1672 Yuseong-daero, Yuseong-gu, Daejeon, 34054, Republic of Korea.
  4. Jeong Hwan Park: Korea Institute of Oriental Medicine (KIOM), 1672 Yuseong-daero, Yuseong-gu, Daejeon, 34054, Republic of Korea.
  5. Dae Wook Kim: Bioresources Industrialization Research Division, Nakdonggang National Institute of Biological Resources, Donam 2-gil 137, Sangju-si, Gyeongsangbuk-do, 37242, Republic of Korea.
  6. Kye Man Cho: Department of Food Science, Institute of Fusion Biotechnology, Gyeongnam National University of Science and Technology, Jinju, 660-758, Republic of Korea.
  7. Seung Mi Kang: Forest Research Department, Gyeongsangnam-do Forest Environment Research Institute, Jingu, 52615, Republic of Korea.
  8. Sang Haeng Choi: AtoGen Co., Ltd., 11-8, Techno 1-ro, Yuseong-gu, Daejeon, 34015, Republic of Korea.
  9. Kun Chul Song: Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
  10. Chung Min Ryu: Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
  11. Keun Chul Lee: Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
  12. Jung-Sook Lee: Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea. jslee@kribb.re.kr.
PMID: 29127501 DOI: 10.1186/s13568-017-0503-1

Abstract

The yeast strain Metschnikowia persimmonesis Kang and Choi et al., sp. nov. [type strain KIOM_G15050 = Korean Collection for Type Cultures (KCTC) 12991BP] was isolated from the stalk of native persimmon cultivars (Diospyros kaki Thumb) obtained from different regions of South Korea and was characterized phenotypically, genetically, and physiologically. The isolate grew between 4 and 40 °C (optimum temperature: 24-28 °C), pH 3-8 (pH optimum = 6.0), and in 0-4% NaCl solution (with optimal growth in absence of NaCl). It also exhibited strong antibiotic and antimicrobial activities. Morphologically, cells were characterized by the presence of long, needle-shaped ascospores. Based on 18S ribosomal DNA gene sequence analysis, the new species was found to belong to the genus Metschnikowia as a sister clade of Metschnikowia fructicola. We therefore conclude that this yeast isolate from D. kaki is a new member of the genus Metschnikowia and propose the name M. persimmonesis sp. nov. This strain has been deposited in the KCTC for future reference. This discovery provides a basis for future research on M. persimmonesis sp. nov., including its possible contribution to the medicinal properties of the host persimmon plant.

Keywords

Diospyros kaki Thumb Korean herbal medicine Metschnikowia persimmonesis sp. nov Persimmon Yeast

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Grants

  1. K17690/Korea Institute of Oriental Medicine
Journal Article
Article has an altmetric score of 2

Word Cloud

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