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01 04, 2018;8 (1): 207-217.

Global Transcriptome Sequencing Reveals Molecular Profiles of Summer Diapause Induction Stage of Onion Maggot, (Diptera: Anthomyiidae).

Shuang Ren, You-Jin Hao, Bin Chen, You-Ping Yin
Author Information
  1. Shuang Ren: Key Lab of Genetic Function and Regulation in Chongqing, School of Life Sciences, Chongqing University, 401331, China.
  2. You-Jin Hao: Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, 401331, China.
  3. Bin Chen: Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, 401331, China.
  4. You-Ping Yin: Key Lab of Genetic Function and Regulation in Chongqing, School of Life Sciences, Chongqing University, 401331, China yinyouping@cqu.edu.cn.
PMID: 29158334 DOI: 10.1534/g3.117.300393

Abstract

The onion maggot, , is a worldwide subterranean pest and can enter diapause during the summer and winter seasons. The molecular regulation of the ontogenesis transition remains largely unknown. Here we used high-throughput RNA sequencing to identify candidate genes and processes linked to summer diapause (SD) induction by comparing the transcriptome differences between the most sensitive larval developmental stage of SD and nondiapause (ND). Nine pairwise comparisons were performed, and significantly differentially regulated transcripts were identified. Several functional terms related to lipid, carbohydrate, and energy metabolism, environmental adaption, immune response, and aging were enriched during the most sensitive SD induction period. A subset of genes, including circadian clock genes, were expressed differentially under diapause induction conditions, and there was much more variation in the most sensitive period of ND- than SD-destined larvae. These expression variations probably resulted in a deep restructuring of metabolic pathways. Potential regulatory elements of SD induction including genes related to lipid, carbohydrate, energy metabolism, and environmental adaption. Collectively, our results suggest the circadian clock is one of the key drivers for integrating environmental signals into the SD induction. Our transcriptome analysis provides insight into the fundamental role of the circadian clock in SD induction in this important model insect species, and contributes to the in-depth elucidation of the molecular regulation mechanism of insect diapause induction.

Keywords

Delia antiqua circadian clock diapause induction genes and pathways transcriptome sequencing

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MeSH Term

Adaptation, Physiological
Animals
CLOCK Proteins
Carbohydrate Metabolism
Circadian Clocks
Diapause, Insect
Diptera
Energy Metabolism
Gene Expression Regulation, Developmental
Gene Ontology
Genome, Insect
High-Throughput Nucleotide Sequencing
Larva
Lipid Metabolism
Molecular Sequence Annotation
Onions
Seasons
Transcriptome

Chemicals

CLOCK Proteins
Journal Article
Article has an altmetric score of 2

Word Cloud

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