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Dec 20, 2017;10 (1): 615.

Molecular identification of Borrelia spirochetes in questing Ixodes ricinus from northwestern Spain.

Pablo Díaz, Jose Luis Arnal, Susana Remesar, Ana Pérez-Creo, José Manuel Venzal, María Esther Vázquez-López, Alberto Prieto, Gonzalo Fernández, Ceferino Manuel López, Rosario Panadero, Alfredo Benito, Pablo Díez-Baños, Patrocinio Morrondo
Author Information
  1. Pablo Díaz: Departamento de Patología Animal (Grupo INVESAGA), Facultad de Veterinaria, Universidade de Santiago de Compostela, Lugo, Spain. pablo.diaz@usc.es.
  2. Jose Luis Arnal: Exopol, Zaragoza, Spain.
  3. Susana Remesar: Departamento de Patología Animal (Grupo INVESAGA), Facultad de Veterinaria, Universidade de Santiago de Compostela, Lugo, Spain.
  4. Ana Pérez-Creo: Departamento de Patología Animal (Grupo INVESAGA), Facultad de Veterinaria, Universidade de Santiago de Compostela, Lugo, Spain.
  5. José Manuel Venzal: Laboratorio de Vectores y enfermedades transmitidas, Facultad de Veterinaria, CENUR Litoral Norte, Universidad de la República, Salto, Uruguay.
  6. María Esther Vázquez-López: Servicio de Pediatría, Hospital Universitario Lucus Augusti, Lugo, Spain.
  7. Alberto Prieto: Departamento de Patología Animal (Grupo INVESAGA), Facultad de Veterinaria, Universidade de Santiago de Compostela, Lugo, Spain.
  8. Gonzalo Fernández: Departamento de Patología Animal (Grupo INVESAGA), Facultad de Veterinaria, Universidade de Santiago de Compostela, Lugo, Spain.
  9. Ceferino Manuel López: Departamento de Patología Animal (Grupo INVESAGA), Facultad de Veterinaria, Universidade de Santiago de Compostela, Lugo, Spain.
  10. Rosario Panadero: Departamento de Patología Animal (Grupo INVESAGA), Facultad de Veterinaria, Universidade de Santiago de Compostela, Lugo, Spain.
  11. Alfredo Benito: Exopol, Zaragoza, Spain.
  12. Pablo Díez-Baños: Departamento de Patología Animal (Grupo INVESAGA), Facultad de Veterinaria, Universidade de Santiago de Compostela, Lugo, Spain.
  13. Patrocinio Morrondo: Departamento de Patología Animal (Grupo INVESAGA), Facultad de Veterinaria, Universidade de Santiago de Compostela, Lugo, Spain.
PMID: 29262835 DOI: 10.1186/s13071-017-2574-x

Abstract

BACKGROUND: Ixodes ricinus, the predominant tick species in Europe, can transmit the causative agents of important human diseases such as Lyme borreliosis (LB), caused by Borrelia spirochetes. In northern Spain, LB is considered endemic; recently, a significant increase of the annual incidence of LB was reported in the northwestern (NW) region.
METHODS: In order to provide information on the prevalence of Borrelia spp., pooled and individually free-living I. ricinus from NW Spain were molecularly analyzed. Positive samples were characterized at the fla and Glpq genes and the rrfA-rrlB intergenic spacer region to identify Borrelia species/genospecies.
RESULTS: Borrelia burgdorferi (sensu lato) (s.l.) individual prevalence and MIR were significantly higher in adult females (32.3 and 16%) than in nymphs (18.8 and 6.2%) and adult males (15.6 and 8.4%). Five Borrelia genospecies belonging to the B. burgdorferi (s.l.) group were identified: B. garinii was predominant, followed by B. valaisiana, B. lusitaniae, B. afzelii and B. burgdorferi (sensu stricto) (s.s.). One species belonging to the tick-borne relapsing fever group (B. miyamotoi) was also found, showing low individual prevalence (1%), positive pool (0.7%) and MIR (0.1%) values. To our knowledge, this is the first citation of B. miyamotoi in free-living ticks from Spain.
CONCLUSIONS: The significant prevalences of B. burgdorferi (s.l.) genospecies detected in questing ticks from NW Spain are similar to those detected in northern and central European countries and higher to those previously found in Spain. These results together with the high incidence of LB in humans and the high seroprevalence of B. burgdorferi (s.l.) in roe deer shown in other studies reveal that the northwest area is one of the most risky regions for acquiring LB in Spain.

Keywords

Borrelia burgdorferi (s.l.) Borrelia miyamotoi Ixodes ricinus NW Spain

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Grants

  1. GRC2015/003/Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia

MeSH Term

Animals
Borrelia burgdorferi Group
Female
Ixodes
Male
Prevalence
Spain
Journal Article
Article has an altmetric score of 23

Word Cloud

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