Towards a more practical attention bias test to assess affective state in sheep.

Jessica E Monk, Rebecca E Doyle, Ian G Colditz, Sue Belson, Greg M Cronin, Caroline Lee
Author Information
  1. Jessica E Monk: Agriculture and Food, CSIRO, Armidale, NSW, Australia. ORCID
  2. Rebecca E Doyle: Animal Welfare Science Centre, University of Melbourne, Parkville, VIC, Australia.
  3. Ian G Colditz: Agriculture and Food, CSIRO, Armidale, NSW, Australia.
  4. Sue Belson: Agriculture and Food, CSIRO, Armidale, NSW, Australia.
  5. Greg M Cronin: Faculty of Science, University of Sydney, Camden, NSW, Australia.
  6. Caroline Lee: Agriculture and Food, CSIRO, Armidale, NSW, Australia.

Abstract

Tests for attention bias potentially offer more rapid assessment of affective state in animals than existing cognitive methods. An attention bias test has previously been developed for sheep and validated as a measure of anxious states. The 3 minute test assessed behavioural responses of sheep in an enclosed arena after brief exposure to the threat of a dog. Experiment 1 of the current study aimed to refine the previously developed method, removing the need for a habituation period and shortening the test duration. Sheep were given either an anxiolytic drug, an anxiogenic drug or a control treatment prior to testing to induce contrasting affective states. Differences in behaviour were found between the treatment groups within the first 45s of the test, indicating the original test duration could be shortened from 180 s. During testing, 36 of 40 animals in the control and anxiolytic groups ate the novel feed offered in the test, indicating it is not necessary to habituate animals to a feed container. Experiment 2 aimed to confirm the responses measured in the test were primarily towards the dog rather than other aspects of the test environment. Sheep exposed to an empty window at the beginning of the test behaved differently to those which were exposed to a dog, indicating sheep behaviour in the test is at least partially a response to the dog. A third group of sheep were also tested with the dog immediately after having small data loggers attached to their necks. Behaviour of these sheep did not differ from the sheep tested without loggers, indicating data logger attachment did not impact their behaviour in the test. In both experiments, treatments did not appear to modify activity (zones crossed), which we propose indicates the test was primarily detecting valence of the affective state rather than arousal.

References

  1. Psychol Rev. 2001 Jul;108(3):483-522 [PMID: 11488376]
  2. Proc Biol Sci. 2010 Oct 7;277(1696):2895-904 [PMID: 20685706]
  3. Biol Lett. 2016 Jun;12 (6): [PMID: 27277950]
  4. Trends Cogn Sci. 1997 Dec;1(9):340-5 [PMID: 21223944]
  5. Pharmacol Biochem Behav. 2004 Mar;77(3):471-9 [PMID: 15006457]
  6. Nature. 2004 Jan 22;427(6972):312 [PMID: 14737158]
  7. Br J Clin Psychol. 1995 Feb;34 ( Pt 1):17-36 [PMID: 7757037]
  8. Biol Lett. 2012 Jun 23;8(3):379-81 [PMID: 22250131]
  9. Genes Brain Behav. 2008 Jun;7(4):463-9 [PMID: 18045243]
  10. J Abnorm Psychol. 1995 Aug;104(3):532-6 [PMID: 7673577]
  11. Behav Res Ther. 2007 Nov;45(11):2664-78 [PMID: 17692819]
  12. Psychopharmacology (Berl). 2014 Feb;231(4):787-800 [PMID: 24114428]
  13. Psychopharmacology (Berl). 2003 May;167(3):274-80 [PMID: 12690422]
  14. Psychol Bull. 2007 Jan;133(1):1-24 [PMID: 17201568]
  15. Psychopharmacology (Berl). 2004 Jul;174(2):190-6 [PMID: 14997269]
  16. J Pers Soc Psychol. 1999 May;76(5):805-19 [PMID: 10353204]
  17. PeerJ. 2015 Dec 14;3:e1510 [PMID: 26713255]
  18. Psychoneuroendocrinology. 2011 Feb;36(2):279-88 [PMID: 20833479]
  19. Behav Res Ther. 1997 Oct;35(10):911-27 [PMID: 9401132]
  20. Psychopharmacology (Berl). 1994 Sep;116(1):120-2 [PMID: 7862925]
  21. PLoS One. 2012;7(8):e44387 [PMID: 22952968]
  22. Neurosci Biobehav Rev. 2005 May;29(3):469-91 [PMID: 15820551]

MeSH Term

Affect
Animal Feed
Animals
Anti-Anxiety Agents
Attention
Behavior, Animal
Dogs
Male
Sheep

Chemicals

Anti-Anxiety Agents

Word Cloud

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