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Blood advances
Aug 08, 2017;1 (18): 1423-1426.

Activated protein C light chain provides an extended binding surface for its anticoagulant cofactor, protein S.

José A Fernández, Xiao Xu, Ranjeet K Sinha, Laurent O Mosnier, Michel F Sanner, John H Griffin
Author Information
  1. José A Fernández: Department of Molecular Medicine and.
  2. Xiao Xu: Department of Molecular Medicine and.
  3. Ranjeet K Sinha: Department of Molecular Medicine and.
  4. Laurent O Mosnier: Department of Molecular Medicine and.
  5. Michel F Sanner: Department of Integrative Structural and Computational Biology, Scripps Research Institute, La Jolla, CA.
  6. John H Griffin: Department of Molecular Medicine and.
PMID: 29296783 DOI: 10.1182/bloodadvances.2017007005

Abstract

Protein S anticoagulant cofactor sensitivity and PAR1 cleavage activity were assayed for 9 recombinant APC mutants.Residues L38, K43, I73, F95, and W115 on one face of the APC light chain define an extended surface containing the protein S binding site.

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Grants

  1. R01 GM096888/NIGMS NIH HHS
  2. R01 HL052246/NHLBI NIH HHS
  3. R01 HL104165/NHLBI NIH HHS
  4. R37 HL052246/NHLBI NIH HHS
Journal Article

Word Cloud

Created with Highcharts 10.0.0SproteinanticoagulantcofactorAPClightchainextendedsurfacebindingProteinsensitivityPAR1cleavageactivityassayed9recombinantmutantsResiduesL38K43I73F95W115onefacedefinecontainingsiteActivatedCprovides

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