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Frontiers in microbiology
2017;8 2659.

Phage Therapy as a Promising New Treatment for Lung Infection Caused by Carbapenem-Resistant in Mice.

Yunfen Hua, Tingting Luo, Yiqi Yang, Dong Dong, Rui Wang, Yanjun Wang, Mengsha Xu, Xiaokui Guo, Fupin Hu, Ping He
Author Information
  1. Yunfen Hua: College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China.
  2. Tingting Luo: College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China.
  3. Yiqi Yang: Department of Medical Microbiology and Immunology, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
  4. Dong Dong: Institute of Antibiotics, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.
  5. Rui Wang: Department of Medical Microbiology and Immunology, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
  6. Yanjun Wang: Department of Medical Microbiology and Immunology, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
  7. Mengsha Xu: College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China.
  8. Xiaokui Guo: Department of Medical Microbiology and Immunology, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
  9. Fupin Hu: Institute of Antibiotics, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.
  10. Ping He: Department of Medical Microbiology and Immunology, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
PMID: 29375524 DOI: 10.3389/fmicb.2017.02659

Abstract

Carbapenem-resistant (CRAB) which is noted as a major pathogen associated with healthcare-associated infections has steadily developed beyond antibiotic control. Lytic bacteriophages with the characteristics of infecting and lysing specific bacteria have been used as a potential alternative to traditional antibiotics to solve multidrug-resistant bacterial infections. Here, we isolated -specific lytic phages and evaluated their potential therapeutic effect against lung infection caused by CRAB clinical strains. The combined lysis spectrum of four lytic phages' ranges was 87.5% (42 of 48) against CRAB clinical isolates. Genome sequence and analysis indicated that phage SH-Ab15519 is a novel phage which does not contain the virulence or antibiotic resistance genes. study indicated that phage SH-Ab15519 administered intranasally can effectively rescue mice from lethal lung infection without deleterious side effects. Our work explores the potential use of phages as an alternative therapeutic agent against the lung infection caused by CRAB strains.

Keywords

bacteriophage carbapenem-resistant Acinetobacter baumannii (CRAB) lung infection mouse model phagotherapy

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