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MicrobiologyOpen
04, 2018;7 (2): e00588.

Quantifying intracellular Mycobacterium tuberculosis: An essential issue for in vitro assays.

Deisy Carolina Rodriguez, Marisol Ocampo, Luz Mary Salazar, Manuel Alfonso Patarroyo
Author Information
  1. Deisy Carolina Rodriguez: Universidad Nacional de Colombia, Bogotá, Colombia.
  2. Marisol Ocampo: Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia. ORCID
  3. Luz Mary Salazar: Universidad Nacional de Colombia, Bogotá, Colombia.
  4. Manuel Alfonso Patarroyo: Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia.
PMID: 29484835 DOI: 10.1002/mbo3.588

Abstract

Many studies about intracellular microorganisms which are important regarding diseases affecting public health have been focused on the recognition of host-pathogen interactions, thereby ascertaining the mechanisms by which the pathogen invades a cell and makes it become its host. Such knowledge enables understanding the immunological response triggered by these interactions for obtaining useful information for developing vaccines and drugs. Quantitative cell infection assay protocols are indispensable regarding studies involving Mycobacterium tuberculosis, which takes the lives of more than 2 million people worldwide every year; however, sometimes these are limited by the pathogen's slow growth. Concerning such limitation, a detailed review is presented here regarding the different methods for quantifying and differentiating an intracellular pathogen, the importance of mycobacteria aggregate dissociation and multiplicity of infection (MOI) in infection assays. The methods' differences, advantages, and disadvantages are discussed regarding intra and extracellular bacteria (on cell surface) differentiation, current problems are outlined, as are the solutions provided using fluorophores and projections made concerning quantitative infection assays.

Keywords

bacterial quantification cytometry fluorescence infection assay tuberculosis

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MeSH Term

Bacterial Load
Flow Cytometry
Fluorescent Antibody Technique
Host-Pathogen Interactions
Humans
Macrophages
Mycobacterium tuberculosis
Polymerase Chain Reaction
Staining and Labeling
Tuberculosis, Pulmonary
Journal Article Review
Article has an altmetric score of 2

Word Cloud

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