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Scientific reports
04 03, 2018;8 (1): 5506.

A comparative analysis of rod bipolar cell transcriptomes identifies novel genes implicated in night vision.

Sasha M Woods, Edward Mountjoy, Duncan Muir, Sarah E Ross, Denize Atan
Author Information
  1. Sasha M Woods: Bristol Medical School, University of Bristol, Bristol, BS8 1TD, UK. Sasha.Woods@bristol.ac.uk.
  2. Edward Mountjoy: Bristol Medical School, University of Bristol, Bristol, BS8 1TD, UK. ORCID
  3. Duncan Muir: Bristol Medical School, University of Bristol, Bristol, BS8 1TD, UK.
  4. Sarah E Ross: Departments of Neurobiology and Anesthesiology and the Center for Pain Research, University of Pittsburgh, Pittsburgh, 15213-2536, USA.
  5. Denize Atan: Bristol Medical School, University of Bristol, Bristol, BS8 1TD, UK.
PMID: 29615777 DOI: 10.1038/s41598-018-23901-6

Abstract

In the mammalian retina, rods and a specialised rod-driven signalling pathway mediate visual responses under scotopic (dim light) conditions. As rods primarily signal to rod bipolar cells (RBCs) under scoptic conditions, disorders that affect rod or RBC function are often associated with impaired night vision. To identify novel genes expressed by RBCs and, therefore, likely to be involved in night vision, we took advantage of the adult Bhlhe23 mouse retina (that lacks RBCs) to derive the RBC transcriptome. We found that genes expressed by adult RBCs are mainly involved in synaptic structure and signalling, whereas genes that influence RBC development are also involved in the cell cycle and transcription/translation. By comparing our data with other published retinal and bipolar cell transcriptomes (where we identify RBCs by the presence of Prkca and/or Pcp2 transcripts), we have derived a consensus for the adult RBC transcriptome. These findings ought to facilitate further research into physiological mechanisms underlying mammalian night vision as well as proposing candidate genes for patients with inherited causes of night blindness.

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MeSH Term

Animals
Biomarkers, Tumor
Gene Expression Profiling
Mice
Nerve Tissue Proteins
Night Vision
Retinal Bipolar Cells
Retinal Rod Photoreceptor Cells

Chemicals

Biomarkers, Tumor
Car8 protein, mouse
Nerve Tissue Proteins
Comparative Study Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 1

Word Cloud

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