OpenLB
Open Library of Bioscience
Advanced Search
Virology
06, 2018;519 12-16.

Novel orthohepeviruses in wild rodents from São Paulo State, Brazil.

William Marciel de Souza, Marilia Farignoli Romeiro, Gilberto Sabino-Santos, Felipe Gonçalves Motta Maia, Marcilio Jorge Fumagalli, Sejal Modha, Marcio Roberto Teixeira Nunes, Pablo Ramiro Murcia, Luiz Tadeu Moraes Figueiredo
Author Information
  1. William Marciel de Souza: Virology Research Center, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900, Monte Alegre, 14049-900 Ribeirão Preto, SP, Brazil; MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom. Electronic address: wmarciel@usp.br.
  2. Marilia Farignoli Romeiro: Virology Research Center, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900, Monte Alegre, 14049-900 Ribeirão Preto, SP, Brazil.
  3. Gilberto Sabino-Santos: Virology Research Center, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900, Monte Alegre, 14049-900 Ribeirão Preto, SP, Brazil.
  4. Felipe Gonçalves Motta Maia: Virology Research Center, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900, Monte Alegre, 14049-900 Ribeirão Preto, SP, Brazil; Laboratory Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
  5. Marcilio Jorge Fumagalli: Virology Research Center, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900, Monte Alegre, 14049-900 Ribeirão Preto, SP, Brazil.
  6. Sejal Modha: MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom.
  7. Marcio Roberto Teixeira Nunes: Center for Technological Innovations, Evandro Chagas Institute, Ministry of Health, Ananindeua, Pará, Brazil.
  8. Pablo Ramiro Murcia: MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom.
  9. Luiz Tadeu Moraes Figueiredo: Virology Research Center, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes, 3900, Monte Alegre, 14049-900 Ribeirão Preto, SP, Brazil.
PMID: 29627586 DOI: 10.1016/j.virol.2018.03.025

Abstract

The Hepeviridae comprise single-stranded positive-sense RNA viruses classified into two genera, Orthohepevirus and Piscihepevirus. orthohepeviruses have a wide host range that includes rodents, but previous studies had been restricted to rodents of the Muridae family. In this study, we applied a high-throughput sequencing approach to examine the presence of orthohepeviruses in rodents from São Paulo State, Brazil. We also used RT-PCR to determine the frequency of orthohepeviruses in our sampled population. We identified novel orthohepeviruses in blood samples derived from Necromys lasiurus (1.19%) and Calomys tener (3.66%). Therefore, our results expand the host range and viral diversity of the Hepeviridae family.

Keywords

Hepatitis E virus Hepeviridae Orthohepevirus Rodent-borne viruses Viral hepatitis

References

  1. BMC Med Res Methodol. 2008 Apr 11;8:20 [PMID: 18405357]
  2. Emerg Infect Dis. 2011 Dec;17(12):2216-22 [PMID: 22172320]
  3. Virology. 2018 Jan 15;514:9-17 [PMID: 29128758]
  4. Emerg Infect Dis. 2012 Aug;18(8):1268-73 [PMID: 22840202]
  5. Mol Biol Evol. 2013 Dec;30(12):2725-9 [PMID: 24132122]
  6. J Infect Dis. 2017 Apr 15;215(8):1197-1206 [PMID: 28199701]
  7. J Gen Virol. 2017 Nov;98(11):2645-2646 [PMID: 29022866]
  8. J Virol. 2013 Jul;87(13):7758-64 [PMID: 23616657]
  9. Nat Methods. 2017 Jun;14(6):587-589 [PMID: 28481363]
  10. Biomed Res Int. 2015;2015:287315 [PMID: 26605326]
  11. Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):1147-1152 [PMID: 28096411]
  12. J Gen Virol. 2014 Oct;95(Pt 10):2223-2232 [PMID: 24989172]
  13. Viruses. 2016 Oct 03;8(10): [PMID: 27706110]
  14. Mol Biol Evol. 2015 Jan;32(1):268-74 [PMID: 25371430]
  15. Infect Genet Evol. 2016 Sep;43:343-6 [PMID: 27282471]
  16. Zoonoses Public Health. 2018 Feb;65(1):11-29 [PMID: 28944602]
  17. Emerg Infect Dis. 2013 Jan;19(1):115-8 [PMID: 23260149]
  18. Nucleic Acids Res. 2004 Mar 19;32(5):1792-7 [PMID: 15034147]
  19. Clin Microbiol Rev. 2014 Jan;27(1):116-38 [PMID: 24396139]

Grants

  1. MC_UU_12014/12/Medical Research Council
  2. MC_UU_12014/9/Medical Research Council
  3. MC_UU_120/14/9/Medical Research Council

MeSH Term

Animals
Animals, Wild
Brazil
Chiroptera
Disease Reservoirs
High-Throughput Nucleotide Sequencing
Host Specificity
Phylogeny
RNA Virus Infections
RNA Viruses
Reverse Transcriptase Polymerase Chain Reaction
Rodent Diseases
Rodentia
Serogroup
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 58

Word Cloud

Created with Highcharts 10.0.0rodentsorthohepevirusesHepeviridaevirusesOrthohepevirushostrangefamilySãoPauloStateBrazilcomprisesingle-strandedpositive-senseRNAclassifiedtwogeneraPiscihepevirusOrthohepeviruseswideincludespreviousstudiesrestrictedMuridaestudyappliedhigh-throughputsequencingapproachexaminepresencealsousedRT-PCRdeterminefrequencysampledpopulationidentifiednovelbloodsamplesderivedNecromyslasiurus119%Calomystener366%ThereforeresultsexpandviraldiversityNovelwildHepatitisEvirusRodent-borneViralhepatitis

Similar Articles

Cited By (9)