In Vivo Bacterial Imaging Using Bioluminescence.

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Mariette Barbier, Justin Bevere, F Heath Damron

Author Information

Mariette Barbier: Department of Microbiology, Immunology and Cell Biology, West Virginia University School of Medicine, Morgantown, WV, USA.
Justin Bevere: Department of Microbiology, Immunology and Cell Biology, West Virginia University School of Medicine, Morgantown, WV, USA.
F Heath Damron: Department of Microbiology, Immunology and Cell Biology, West Virginia University School of Medicine, Morgantown, WV, USA. fdamron@hsc.wvu.edu.

PMID: 29858785 DOI: 10.1007/978-1-4939-7860-1_7

Bacterial luminescence allows for noninvasive continuous monitoring of promoter activity in a wide range of model systems. This chapter details various examples of use of the lux reporter system to measure promoter activity in bacteria using the vector pUC18T-mini-Tn7T-lux-Tp. Here, we describe the construction of promoter fusions with bacterial luciferase, and how to quantify promoter activity in real time in vitro and in vivo in plant, insect, and murine infection models.

Bacteria Bacterial pathogenesis Bioluminescence In vivo imaging Pathogen-host interaction Promoter activity Reporter system

Animals

Bacteria

Insecta

Luciferases

Luminescence

Luminescent Measurements

Mice

Molecular Imaging

Plants

Plasmids

Promoter Regions, Genetic

Luciferases

Journal Article

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