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Nucleic acids research
10 12, 2018;46 (18): e111.

A universal gene expression system for fungi.

Anssi Rantasalo, Christopher P Landowski, Joosu Kuivanen, Annakarin Korppoo, Lauri Reuter, Outi Koivistoinen, Mari Valkonen, Merja Penttilä, Jussi Jäntti, Dominik Mojzita
Author Information
  1. Anssi Rantasalo: VTT Technical Research Centre of Finland, Espoo, P.O. Box 1000, FI-02044 VTT, Finland.
  2. Christopher P Landowski: VTT Technical Research Centre of Finland, Espoo, P.O. Box 1000, FI-02044 VTT, Finland.
  3. Joosu Kuivanen: VTT Technical Research Centre of Finland, Espoo, P.O. Box 1000, FI-02044 VTT, Finland.
  4. Annakarin Korppoo: VTT Technical Research Centre of Finland, Espoo, P.O. Box 1000, FI-02044 VTT, Finland.
  5. Lauri Reuter: VTT Technical Research Centre of Finland, Espoo, P.O. Box 1000, FI-02044 VTT, Finland.
  6. Outi Koivistoinen: VTT Technical Research Centre of Finland, Espoo, P.O. Box 1000, FI-02044 VTT, Finland.
  7. Mari Valkonen: VTT Technical Research Centre of Finland, Espoo, P.O. Box 1000, FI-02044 VTT, Finland.
  8. Merja Penttilä: VTT Technical Research Centre of Finland, Espoo, P.O. Box 1000, FI-02044 VTT, Finland.
  9. Jussi Jäntti: VTT Technical Research Centre of Finland, Espoo, P.O. Box 1000, FI-02044 VTT, Finland.
  10. Dominik Mojzita: VTT Technical Research Centre of Finland, Espoo, P.O. Box 1000, FI-02044 VTT, Finland.
PMID: 29924368 DOI: 10.1093/nar/gky558

Abstract

Biotechnological production of fuels, chemicals and proteins is dependent on efficient production systems, typically genetically engineered microorganisms. New genome editing methods are making it increasingly easy to introduce new genes and functionalities in a broad range of organisms. However, engineering of all these organisms is hampered by the lack of suitable gene expression tools. Here, we describe a synthetic expression system (SES) that is functional in a broad spectrum of fungal species without the need for host-dependent optimization. The SES consists of two expression cassettes, the first providing a weak, but constitutive level of a synthetic transcription factor (sTF), and the second enabling strong, at will tunable expression of the target gene via an sTF-dependent promoter. We validated the SES functionality in six yeast and two filamentous fungi species in which high (levels beyond organism-specific promoters) as well as adjustable expression levels of heterologous and native genes was demonstrated. The SES is an unprecedentedly broadly functional gene expression regulation method that enables significantly improved engineering of fungi. Importantly, the SES system makes it possible to take in use novel eukaryotic microbes for basic research and various biotechnological applications.

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MeSH Term

Aspergillus niger
Cloning, Molecular
Fungi
Gene Expression
Gene Expression Regulation, Fungal
Genetic Engineering
Genetic Vectors
Recombinant Proteins
Saccharomyces cerevisiae
Synthetic Biology
Trichoderma

Chemicals

Recombinant Proteins
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 29

Word Cloud

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