Evaluation of cultivated and wild genotypes of Lens species under alkalinity stress and their molecular collocation using microsatellite markers.

Dharmendra Singh, Chandan Kumar Singh, Yash Pal Singh, Vijayata Singh, Rajendra Singh, Ram Sewak Singh Tomar, Satish Kumar Sanwal, Sourabh Karwa, Vinay Kumar Mishra, Susheel Kumar Sarkar, Madan Pal, Arun Kumar, Rajendra Kumar Yadav, Parbodh Chander Sharma
Author Information
  1. Dharmendra Singh: Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India. ORCID
  2. Chandan Kumar Singh: Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, India.
  3. Yash Pal Singh: ICAR-Regional Station of Central Soil Salinity Research Institute, Lucknow, India.
  4. Vijayata Singh: ICAR- Central Soil Salinity Research Institute, Karnal, India.
  5. Rajendra Singh: Divisions of Soil Science and Agricultural Chemistry, ICAR-Indian Agricultural Research Institute, New Delhi, India.
  6. Ram Sewak Singh Tomar: ICAR-National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, India.
  7. Satish Kumar Sanwal: ICAR- Central Soil Salinity Research Institute, Karnal, India.
  8. Sourabh Karwa: Division of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi, India.
  9. Vinay Kumar Mishra: ICAR-Regional Station of Central Soil Salinity Research Institute, Lucknow, India.
  10. Susheel Kumar Sarkar: ICAR-Indian Agricultural Statistical Research Institute, New Delhi, India.
  11. Madan Pal: Division of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi, India.
  12. Arun Kumar: National Phytotron Facility, ICAR-Indian Agricultural Research Institute, New Delhi, India.
  13. Rajendra Kumar Yadav: Department of Genetics and Plant Breeding, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur, India.
  14. Parbodh Chander Sharma: ICAR- Central Soil Salinity Research Institute, Karnal, India.

Abstract

In this study, 285 lentil genotypes were phenotyped under hydroponic and alkaline field conditions. Significant genotypic variation for alkalinity stress was observed among the six Lens species screened hydroponically and in the field having pH up to 9.1. The crucial parameters, like whole Na+ and K+ contents and the Na+/K+ ratio at 40 mM NaHCO3 were found significantly correlated with seedling survivability under hydroponics (r = -0.95, r = 0.93 and -0.97). Genotypes, ranked on the bases of seed yield, restricted uptake of Na+ with thick pith area, increased vascular bundles, less H2O2 production and low Na+/K+ ratio, were found important physio-anatomical traits for alkalinity stress tolerance. The proper regulation of Na+ uptake was found for maintaining higher K+. This relationship is probably the main factor responsible for a better mechanism for tolerance to high pH up to 9.1 in tolerant breeding lines PDL-1 and PSL-9 (cultivars) and ILWL-15, ILWL-192 and ILWL-20 (wild accessions). Based on UPGMA dendrogram, all the genotypes were clustered into four diverse groups. DMRT was implied within the group to differentiate genotypes based on phenotypic response under alkalinity stress. These results can be utilized for selecting diverse parents for developing alkalinity tolerant genotypes.

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MeSH Term

Cations, Monovalent
Genotype
Hydrogen-Ion Concentration
Hydroponics
Lens Plant
Microsatellite Repeats
Plant Breeding
Plant Roots
Potassium
Sodium
Species Specificity
Stress, Physiological

Chemicals

Cations, Monovalent
Sodium
Potassium

Word Cloud

Created with Highcharts 10.0.0genotypesalkalinitystressNa+foundfieldLensspeciespH91K+Na+/K+ratior=-0uptaketolerancetolerantwilddiversestudy285lentilphenotypedhydroponicalkalineconditionsSignificantgenotypicvariationobservedamongsixscreenedhydroponicallycrucialparameterslikewholecontents40mMNaHCO3significantlycorrelatedseedlingsurvivabilityhydroponics9509397GenotypesrankedbasesseedyieldrestrictedthickpithareaincreasedvascularbundleslessH2O2productionlowimportantphysio-anatomicaltraitsproperregulationmaintaininghigherrelationshipprobablymainfactorresponsiblebettermechanismhighbreedinglinesPDL-1PSL-9cultivarsILWL-15ILWL-192ILWL-20accessionsBasedUPGMAdendrogramclusteredfourgroupsDMRTimpliedwithingroupdifferentiatebasedphenotypicresponseresultscanutilizedselectingparentsdevelopingEvaluationcultivatedmolecularcollocationusingmicrosatellitemarkers

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