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Sep 04, 2018;51 (1): 30.

Knockdown of lncRNA MIR31HG inhibits cell proliferation in human HaCaT keratinocytes.

Jintao Gao, Fangru Chen, Mingchun Hua, Junfan Guo, Yuejuan Nong, Qinyan Tang, Fengxia Zhong, Linxiu Qin
Author Information
  1. Jintao Gao: College of Biotechnology, Guilin Medical University, Guilin, 541100, Guangxi, People's Republic of China. jintao_gao@glmc.edu.cn. ORCID
  2. Fangru Chen: Department of Dermatology, Affiliated Hospital of Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China.
  3. Mingchun Hua: Department of Plastic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China.
  4. Junfan Guo: College of Biotechnology, Guilin Medical University, Guilin, 541100, Guangxi, People's Republic of China.
  5. Yuejuan Nong: College of Biotechnology, Guilin Medical University, Guilin, 541100, Guangxi, People's Republic of China.
  6. Qinyan Tang: College of Biotechnology, Guilin Medical University, Guilin, 541100, Guangxi, People's Republic of China.
  7. Fengxia Zhong: College of Biotechnology, Guilin Medical University, Guilin, 541100, Guangxi, People's Republic of China.
  8. Linxiu Qin: College of Biotechnology, Guilin Medical University, Guilin, 541100, Guangxi, People's Republic of China.
PMID: 30180891 DOI: 10.1186/s40659-018-0181-8

Abstract

BACKGROUND: Psoriasis is a complex, chronic inflammatory skin disease with substantial negative effects on patient quality of life. Long non-coding RNAs (lncRNAs) are able to be involved in multitudes of cellular processes in diverse human diseases. This study aimed to investigate the potential involvement of lncRNA MIR31HG in HaCaT keratinocytes proliferation.
RESULTS: The study showed that MIR31HG was significantly elevated in the lesional psoriatic skin compared with normal individuals' skin. Knockdown of MIR31HG inhibited HaCaT keratinocytes proliferation. Flow cytometry analysis showed that siRNA-mediated MIR31HG depletion induced cell cycle arrest in the G2/M phase. In addition, MIR31HG expression was found to be dependent on NF-κB activation.
CONCLUSIONS: NF-κB activation mediated MIR31HG upregulation plays an important role in the regulation of HaCaT keratinocytes proliferation. It could be a potential diagnostic biomarker and therapeutic target for psoriasis.

Keywords

HaCaT keratinocytes Knockdown LncRNA MIR31HG Proliferation

References

  1. Int J Mol Sci. 2012 Dec 21;14(1):205-25 [PMID: 23344029]
  2. J Immunol. 2010 Mar 24;184(9):5263-5270 [PMID: 20335534]
  3. Oncogene. 2015 Jul;34(27):3493-503 [PMID: 25174402]
  4. Nat Commun. 2015 Jul 03;6:7652 [PMID: 26138368]
  5. Nature. 2007 Feb 22;445(7130):866-73 [PMID: 17314973]
  6. Nat Rev Dis Primers. 2016 Nov 24;2:16082 [PMID: 27883001]
  7. J Invest Dermatol. 1990 Oct;95(4):450-5 [PMID: 1698888]
  8. Br J Dermatol. 2006 Oct;155(4):688-94 [PMID: 16965416]
  9. Genome Biol. 2015 Jan 30;16:24 [PMID: 25723451]
  10. Exp Cell Res. 2018 Feb 15;363(2):243-254 [PMID: 29339075]
  11. Exp Dermatol. 2010 Mar;19(3):269-78 [PMID: 20377629]
  12. Oncogene. 2016 Jul 14;35(28):3647-57 [PMID: 26549028]
  13. Arch Dermatol Res. 1991;283(7):465-71 [PMID: 1724897]
  14. J Biol Chem. 2005 Jun 24;280(25):24159-67 [PMID: 15855153]
  15. Br J Dermatol. 1995 Oct;133(4):501-11 [PMID: 7577575]
  16. J Exp Clin Cancer Res. 2015 Sep 11;34:100 [PMID: 26362196]
  17. Cell Prolif. 2017 Dec;50(6):null [PMID: 28963737]
  18. Stem Cell Res. 2017 Dec;25:88-97 [PMID: 29125994]
  19. N Engl J Med. 2009 Jul 30;361(5):496-509 [PMID: 19641206]
  20. Trends Immunol. 2004 Jun;25(6):280-8 [PMID: 15145317]
  21. Trends Cell Biol. 2011 Jun;21(6):354-61 [PMID: 21550244]
  22. Nat Commun. 2017 Jun 22;8:15874 [PMID: 28639619]
  23. Nat Rev Genet. 2009 Mar;10(3):155-9 [PMID: 19188922]
  24. J Mol Biol. 2013 Oct 9;425(19):3731-46 [PMID: 23467124]
  25. J Invest Dermatol. 2016 Mar;136(3):603-9 [PMID: 27015450]

Grants

  1. 2016GXNSFBA380146/Natural Science Foundation of Guangxi Zhuang Autonomous Region
  2. KY2016YB329/Project of Improving the Basic Ability of Young and Middle-aged Teachers of Guangxi Zhuang Autonomous Region

MeSH Term

Biomarkers
Case-Control Studies
Cell Proliferation
Gene Expression Regulation
Humans
Keratinocytes
Psoriasis
RNA, Long Noncoding
Signal Transduction
Up-Regulation

Chemicals

Biomarkers
RNA, Long Noncoding
Journal Article
Article has an altmetric score of 1

Word Cloud

Created with Highcharts 10.0.0MIR31HGHaCaTkeratinocytesproliferationskinKnockdownhumanstudypotentiallncRNAshowedcellNF-κBactivationBACKGROUND:PsoriasiscomplexchronicinflammatorydiseasesubstantialnegativeeffectspatientqualitylifeLongnon-codingRNAslncRNAsableinvolvedmultitudescellularprocessesdiversediseasesaimedinvestigateinvolvementRESULTS:significantlyelevatedlesionalpsoriaticcomparednormalindividuals'inhibitedFlowcytometryanalysissiRNA-mediateddepletioninducedcyclearrestG2/MphaseadditionexpressionfounddependentCONCLUSIONS:mediatedupregulationplaysimportantroleregulationdiagnosticbiomarkertherapeutictargetpsoriasisinhibitsLncRNAProliferation

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