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Scientific reports
09 06, 2018;8 (1): 13315.

Impaired microcirculation after subarachnoid hemorrhage in an in vivo animal model.

Kuo-Chuan Wang, Sung-Chun Tang, Jing-Er Lee, Jui-Chang Tsai, Dar-Ming Lai, Wei-Chou Lin, Chih-Peng Lin, Yong-Kwang Tu, Sung-Tsang Hsieh
Author Information
  1. Kuo-Chuan Wang: Division of Neurosurgery, Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan.
  2. Sung-Chun Tang: Department of Neurology, National Taiwan University Hospital, Taipei, Taiwan.
  3. Jing-Er Lee: Department of Neurology, Taipei Medical University-Wan Fang Hospital, Taipei, Taiwan.
  4. Jui-Chang Tsai: Division of Neurosurgery, Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan.
  5. Dar-Ming Lai: Division of Neurosurgery, Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan.
  6. Wei-Chou Lin: Department of Pathology, National Taiwan University Hospital, Taipei, Taiwan.
  7. Chih-Peng Lin: Department of Anesthesiology, National Taiwan University Hospital, Taipei, Taiwan. ORCID
  8. Yong-Kwang Tu: Division of Neurosurgery, Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan.
  9. Sung-Tsang Hsieh: Department of Neurology, National Taiwan University Hospital, Taipei, Taiwan. shsieh@ntu.edu.tw. ORCID
PMID: 30190518 DOI: 10.1038/s41598-018-31709-7

Abstract

The influence of aneurysmal subarachnoid hemorrhage (SAH) on brain microcirculation has not yet been systematically investigated. We established an animal model to examine (1) the brain surface microcirculation (2) the influences of cerebrospinal fluid (CSF) from aneurysmal SAH on the brain surface microcirculation. A rat SAH model was induced by injection of autologous arterial blood into the cisterna magnum, and the brain surface microcirculation was evaluated by a capillary videoscope with craniotomy at the fronto-parietal region. CSF from SAH rats and SAH patients was applied on the brain surface of naïve rats to assess the resulting microcirculatory changes. In the SAH rats, diffuse constriction of cortical arterioles within 24 hours of SAH was observed. Similar patterns of microcirculation impairment were induced on normal rat brain surfaces via application of CSF from SAH rats and SAH patients. Furthermore, the proportion of subjects with arteriolar vasoconstriction was significantly higher in the group of SAH patients with delayed ischemic neurological deficits (DIND) than in those without DIND (p < 0.001). This study demonstrated impaired microcirculation on brain surface arterioles in a rat model of SAH. CSF from SAH rats and patients was responsible for impairment of brain surface microcirculation.

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Grants

  1. NTUH.102-M2313/National Taiwan University Hospital (NTUH)

MeSH Term

Animals
Brain
Cerebrovascular Circulation
Disease Models, Animal
Male
Microcirculation
Rats
Rats, Wistar
Subarachnoid Hemorrhage
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 1

Word Cloud

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