Xingdong Yang: Institute of Food and Drug Inspection, Zhoukou Normal University, Zhoukou 466001, People's Republic of China. ORCID
Zhongke Sun: Institute of Food and Drug Inspection, Zhoukou Normal University, Zhoukou 466001, People's Republic of China.
Fengshou Tian: Institute of Food and Drug Inspection, Zhoukou Normal University, Zhoukou 466001, People's Republic of China.
Guochao Jia: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, People's Republic of China.
Jifei Yang: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, People's Republic of China.
Xiaofei Hu: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, People's Republic of China.
A lateral flow immunochromatographic strip test was developed for rapid and sensitive on-site detection of hexoestrol (HES) residues in fish samples with colloidal gold labelling of the anti-HES monoclonal antibody. The strip is composed of a sample pad, a conjugate reagent pad, an absorbent pad and a test membrane containing a control line and a test line. The sensitivity (half inhibitory concentration, IC) of the strip in the detection of fish extract samples was confirmed to be 1.86 µg kg, and the limit of detection value was 0.62 µg kg. For intra-assay and inter-assay reproducibility, recoveries of HES-spiked samples ranged from 86.3% to 92.3% and 85.8% to 93.4%, coefficients of variation were 2.91-4.64% and 4.24-5.17%, respectively. High-performance liquid chromatography was employed to confirm the performance of the strip. The strip test takes less than 10 min, and thus provides a repaid method for on-site detection of HES residues.
