Silver using leaf extract: apoptosis induction in MCF-7 breast cancer cell line.

Shima Edalat Fard, Farzaneh Tafvizi, Maryam Bikhof Torbati
Author Information
  1. Shima Edalat Fard: Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran.
  2. Farzaneh Tafvizi: Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran. farzanehtafvizi54@gmail.com.
  3. Maryam Bikhof Torbati: Department of Biology, College of Science, Yadegar-e-Imam Khomeini (RAH), Shahr-e-Rey Branch, Islamic Azad University, Tehran, Iran.

Abstract

The aim of this study was to green synthesised silver nanoparticles (AgNPs) using leaf extract and investigate the cytotoxic and apoptosis-inducing effects of these nanoparticles in MCF-7 breast cancer cell line. The characteristics and morphology of the green synthesised AgNPs were evaluated using transmission electron microscopy, scanning electron microscopy, UV-visible spectroscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy. The MTT assay was used to investigate the anti-proliferative activity of biosynthesised nanoparticles in MCF-7 cells. Apoptosis test was performed using flow cytometry and expression of caspase 3 and 9 genes. The spherical AgNPs with an average size of 19.17 nm were synthesised. The results showed that biosynthesised AgNPs exhibited cytotoxicity, anti-cancer, apoptosis induction, and increased expression of genes encoding for caspases 3 and 9 in MCF-7 cancer cells in a concentration- and time-dependent manner. It seems that green synthesised AgNPs have potential uses for pharmaceutical industries.

References

  1. Colloids Surf B Biointerfaces. 2012 Mar 1;91:212-4 [PMID: 22119564]
  2. Artif Cells Nanomed Biotechnol. 2018;46(sup1):499-510 [PMID: 29361855]
  3. J Nucl Med Technol. 2011 Dec;39(4):279-83 [PMID: 22080437]
  4. Nano Lett. 2005 May;5(5):829-34 [PMID: 15884879]
  5. J Clin Biochem Nutr. 2007 Jul;41(1):1-11 [PMID: 18392106]
  6. Chem Phys Lett. 2010 Feb 25;487(1-3): [PMID: 24187379]
  7. ACS Nano. 2008 Oct 28;2(10):2121-34 [PMID: 19206459]
  8. Breast Cancer (Auckl). 2015 Apr 15;9:9-17 [PMID: 25922577]
  9. Cancer Nanotechnol. 2011;2(1-6):57-65 [PMID: 26069485]
  10. Toxicol Lett. 2008 Jul 10;179(3):130-9 [PMID: 18547751]
  11. Biomed Chromatogr. 2006 Feb;20(2):151-3 [PMID: 16206136]
  12. Colloids Surf B Biointerfaces. 2009 Oct 15;73(2):332-8 [PMID: 19576733]
  13. Chem Pharm Bull (Tokyo). 2001 Oct;49(10):1368-71 [PMID: 11605675]
  14. Ecotoxicol Environ Saf. 2016 Dec;134(Pt 2):308-318 [PMID: 26277620]
  15. Colloids Surf B Biointerfaces. 2012 Jun 1;94:226-30 [PMID: 22348986]
  16. Afr J Tradit Complement Altern Med. 2008 Oct 25;6(1):9-16 [PMID: 20162036]
  17. J Nanobiotechnology. 2013 Jul 19;11:26 [PMID: 23870291]
  18. Res Pharm Sci. 2014 Nov-Dec;9(6):385-406 [PMID: 26339255]
  19. J Phys Chem B. 2008 Oct 30;112(43):13608-19 [PMID: 18831567]
  20. J Nanobiotechnology. 2005 Jun 29;3:6 [PMID: 15987516]
  21. Nano Lett. 2006 Aug;6(8):1794-807 [PMID: 16895376]
  22. J Pharmacol Exp Ther. 2005 Apr;313(1):333-44 [PMID: 15626723]
  23. Phytomedicine. 2000 Oct;7(5):427-48 [PMID: 11081995]
  24. Environ Sci Technol. 2007 Jul 15;41(14):5137-42 [PMID: 17711235]

MeSH Term

Antineoplastic Agents
Apoptosis
Cell Survival
Centella
Green Chemistry Technology
Humans
MCF-7 Cells
Metal Nanoparticles
Plant Extracts
Plant Leaves
Silver

Chemicals

Antineoplastic Agents
Plant Extracts
Silver

Word Cloud

Created with Highcharts 10.0.0AgNPssynthesisedusingMCF-7greennanoparticlescancerleafinvestigatebreastcelllineelectronmicroscopyspectroscopybiosynthesisedcellsexpression39genesapoptosisinductionaimstudysilverextractcytotoxicapoptosis-inducingeffectscharacteristicsmorphologyevaluatedtransmissionscanningUV-visibleX-raydiffractionFourier-transforminfraredMTTassayusedanti-proliferativeactivityApoptosistestperformedflowcytometrycaspasesphericalaveragesize1917 nmresultsshowedexhibitedcytotoxicityanti-cancerincreasedencodingcaspasesconcentration-time-dependentmannerseemspotentialusespharmaceuticalindustriesSilverextract:

Similar Articles

Cited By