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Cell adhesion & migration
12, 2019;13 (1): 83-97.

Protein kinase C Inhibitors selectively modulate dynamics of cell adhesion molecules and cell death in human colon cancer cells.

Muzaffer Dükel, Zehra Tavsan, Duygu Erdogan, Deniz Erkan Gök, Hulya Ayar Kayali
Author Information
  1. Muzaffer Dükel: a Moleculer Biology and Genetic Department, Faculty of Art and Science , Mehmet Akif Ersoy University , Burdur , Turkey.
  2. Zehra Tavsan: b Izmir Biomedicine and Genome Center , Izmir , Turkey.
  3. Duygu Erdogan: c Izmir International Biomedicine and Genome Institute , Dokuz Eylül University , Izmir , Turkey.
  4. Deniz Erkan Gök: b Izmir Biomedicine and Genome Center , Izmir , Turkey.
  5. Hulya Ayar Kayali: b Izmir Biomedicine and Genome Center , Izmir , Turkey.
PMID: 30289336 DOI: 10.1080/19336918.2018.1530933

Abstract

During development of colon cancer, Protein Kinase Cs (PKCs) are involved in regulation of many genes controlling several cellular mechanisms. Here, we examined the changes in cell adhesion molecules and PKCs for colorectal cancer progression. We identified that PKCs affected expression of EpCAM, claudins, tetraspanins. Treatment with low concentrations of PKC inhibitors resulted in decreased cell viability. In addition, immunoblotting and qRT-PCR analysis showed that apoptosis was inhibited while autophagy was induced by PKC inhibition in colon cancer cells. Furthermore, we observed decreased levels of intracellular Reactive Oxygen Species (ROS), lipid peroxidation and protein carbonyl, confirming the ROS-induced apoptosis. Taken together, our results reveal that PKC signalling modulates not only cell adhesion dynamics but also cell death-related mechanisms. Abbreviations: PKC: Protein Kinase C; EpCAM: Epithelial cell adhesion molecule; FBS: fetal bovine serum; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide); CAM: cell adhesion molecule; ROS: reactive oxygen species.

Keywords

Colon cancer cell adhesion molecules cell death protein kinase C reactive oxygen species

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MeSH Term

Apoptosis
Autophagy
Cell Adhesion
Cell Adhesion Molecules
Collagen
Colonic Neoplasms
Gene Expression Regulation, Neoplastic
Humans
Protein Kinase C
Protein Kinase Inhibitors
Reactive Oxygen Species
Tumor Cells, Cultured

Chemicals

Cell Adhesion Molecules
Protein Kinase Inhibitors
Reactive Oxygen Species
Collagen
Protein Kinase C
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 1

Word Cloud

Created with Highcharts 10.0.0celladhesioncancercolonProteinPKCsmoleculesPKCCKinasemechanismsdecreasedapoptosiscellsproteindynamicsmoleculereactiveoxygenspecieskinasedeathdevelopmentCsinvolvedregulationmanygenescontrollingseveralcellularexaminedchangescolorectalprogressionidentifiedaffectedexpressionEpCAMclaudinstetraspaninsTreatmentlowconcentrationsinhibitorsresultedviabilityadditionimmunoblottingqRT-PCRanalysisshowedinhibitedautophagyinducedinhibitionFurthermoreobservedlevelsintracellularReactiveOxygenSpeciesROSlipidperoxidationcarbonylconfirmingROS-inducedTakentogetherresultsrevealsignallingmodulatesalsodeath-relatedAbbreviations:PKC:EpCAM:EpithelialFBS:fetalbovineserumMTT:3-45-dimethylthiazol-2-yl-25-diphenyltetrazoliumbromideCAM:ROS:InhibitorsselectivelymodulatehumanColon

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