An underutilized residue called Citrus Pulp of Floater (CPF), which causes problems during the industrial process of manufacturing of orange juice, was explored for the production of high value-added products. Mixed, first (1G) and second generation (2G) ethanol, a clean and renewable biofuel, was obtained after an enzyme cocktail isolated from the Xanthomonas axonopodis pv. citri (Xac) was applied in hydrolysis of this biomass. Then, mono- and co-culture fermentations were performed using the yeast Saccharomyces cerevisiae and two Candida strains (Candida parapsilosis IFM 48375 and NRRL Y-12969), where the last two were isolated from the orange bagasse in natura. After the enzymatic hydrolysis step, sugars obtained were converted to ethanol achieving a yield of almost 100% after co-fermentation. Hesperidin, a flavonoid widely used for its antimicrobial and/or antioxidant activities, was also extracted from CPF by liquid-solid extraction and precipitation, with the yield of 1.2% and 92.6% pure. Finally, nanocellulose was produced through processes such as extraction, bleaching and nanonization with the yield of 1.4% and over 98% of purity. These products - ethanol, Hesperidin and nanocellulose were obtained from three independent processes: (1) after an enzyme-based hydrolysis of CPF, liquid part was used for ethanol production, and solid was preserved; (2) Hesperidin was isolated from a dry CPF, and solid residue was preserved; and (3) nanocellulose was obtained from the solid residues after processes cited in 1 and 2.
