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Methods in molecular biology (Clifton, N.J.)
2019;1875 21-36.

Phytoplasma Transmission: Insect Rearing and Infection Protocols.

L Pagliari, J Chuche, D Bosco, D Thiéry
Author Information
  1. L Pagliari: Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy. laura.pagliari@uniud.it.
  2. J Chuche: IFV, Pôle Nouvelle Aquitaine, Blanquefort, France.
  3. D Bosco: Department of Agriculture, Forestry and Food Sciences, University of Torino, Grugliasco, Italy.
  4. D Thiéry: INRA, UMR 1065 Save "Santé et Agroécologie du VignoblE", Villenave d'Ornon, France.
PMID: 30361993 DOI: 10.1007/978-1-4939-8837-2_3

Abstract

Phytoplasmas are obligate pathogens and thus they can be studied only in association with their plants or insect hosts. In this chapter, we present protocols for rearing some phytoplasma insect vectors, to obtain infected insects and plants under controlled environmental conditions. We focus on Euscelidius variegatus and Macrosteles quadripunctulatus that can infect Arabidopsis thaliana, and Hyalesthes obsoletus and Scaphoideus titanus, that can infect grapevine.

Keywords

Euscelidius variegatus Hyalesthes obsoletus Infection protocol Macrosteles quadripunctulatus Scaphoideus titanus

MeSH Term

Animals
Arabidopsis
Hemiptera
Herbivory
Insect Vectors
Phytoplasma
Plant Diseases
Journal Article
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