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Dec, 2018;43 (12): 2404-2422.

Neuroprotective Effect of Anethole Against Neuropathic Pain Induced by Chronic Constriction Injury of the Sciatic Nerve in Mice.

Bing Wang, Guoxin Zhang, Mei Yang, Ning Liu, Yu-Xiang Li, Hanxiang Ma, Lin Ma, Tao Sun, Huanran Tan, Jianqiang Yu
Author Information
  1. Bing Wang: Department of Pharmacology, College of Pharmacy, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, 750004, People's Republic of China.
  2. Guoxin Zhang: Department of Pharmacology, College of Pharmacy, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, 750004, People's Republic of China.
  3. Mei Yang: Department of Pharmacology, College of Pharmacy, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, 750004, People's Republic of China.
  4. Ning Liu: Department of Pharmacology, College of Pharmacy, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, 750004, People's Republic of China.
  5. Yu-Xiang Li: College of Nursing, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, 750004, People's Republic of China.
  6. Hanxiang Ma: Department of Anesthesiology, General Hospital of Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, 750004, People's Republic of China.
  7. Lin Ma: Ningxia Key Laboratory of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, 750004, People's Republic of China.
  8. Tao Sun: Ningxia Key Laboratory of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, 750004, People's Republic of China.
  9. Huanran Tan: Department of Pharmacology, Peking University, Health Science Center, Beijing, 100191, People's Republic of China. tanlab@bjmu.edu.cn.
  10. Jianqiang Yu: Department of Pharmacology, College of Pharmacy, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region, 750004, People's Republic of China. YujqLab@163.com.
PMID: 30367337 DOI: 10.1007/s11064-018-2668-7

Abstract

Neuropathic pain is an intractable disease with few definitive therapeutic options. Anethole (AN) has been confirmed to possess potent anti-inflammatory and neuroprotective properties, but its effect on neuropathic pain has not been reported. The present study was designed to investigate the antinociceptive effect of AN on chronic constriction injury (CCI)-induced neuropathic pain in mice. AN (125, 250, and 500 mg/kg) and pregabalin (40 mg/kg) were intragastric administered for 8 consecutive days from the 7th day post-surgery. Behavioral parameters were measured on different days, namely, 0, 7, 8, 10, 12, and 14, from CCI operation. Additionally, electrophysiological and histopathological changes were analyzed on the 14th day. Afterward, immunofluorescence and Western blot were utilized to examine the activation of glial cells and the expression of inflammatory cytokines, respectively. AN treatment of CCI mice considerably alleviated hyperalgesia and allodynia, ameliorated abnormal sciatic nerve conduction, and restored injured sciatic nerves in a dose-dependent manner. Furthermore, AN suppressed the activation of glial cells, down-regulated pro-inflammatory cytokines such as tumor necrosis factor alpha (TNF-α), interleukin (IL-6, and IL-1β), and up-regulated the anti-inflammatory cytokine (IL-10). These assays first indicated that AN exerted an antinociceptive effect on CCI-induced neuropathic pain, and might be attributed to the anti-inflammatory and neuroprotective activities of AN.

Keywords

Anethole Behavioral tests Chronic constriction injury Inflammation Neuropathic pain Neuroprotection

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Grants

  1. 81360182/National Natural Science Foundation of China
  2. 2017BY079/Key Research and Development Project in Ningxia Hui Autonomous Region
  3. 2016BZ07/The "13th Five-Year Plan" Major Science and Technology Project in Ningxia Hui Autonomous Region

MeSH Term

Allylbenzene Derivatives
Animals
Anisoles
Constriction
Male
Mice
Mice, Inbred ICR
Neuralgia
Neuroprotective Agents
Sciatic Neuropathy

Chemicals

Allylbenzene Derivatives
Anisoles
Neuroprotective Agents
anethole
Journal Article

Word Cloud

Created with Highcharts 10.0.0ANpainNeuropathicAnetholeanti-inflammatoryeffectneuropathicCCIneuroprotectiveantinociceptiveconstrictioninjurymice8daysdayBehavioralactivationglialcellscytokinessciaticChronicintractablediseasedefinitivetherapeuticoptionsconfirmedpossesspotentpropertiesreportedpresentstudydesignedinvestigatechronic-induced125250500 mg/kgpregabalin40 mg/kgintragastricadministeredconsecutive7thpost-surgeryparametersmeasureddifferentnamely07101214operationAdditionallyelectrophysiologicalhistopathologicalchangesanalyzed14thAfterwardimmunofluorescenceWesternblotutilizedexamineexpressioninflammatoryrespectivelytreatmentconsiderablyalleviatedhyperalgesiaallodyniaamelioratedabnormalnerveconductionrestoredinjurednervesdose-dependentmannerFurthermoresuppresseddown-regulatedpro-inflammatorytumornecrosisfactoralphaTNF-αinterleukinIL-6IL-1βup-regulatedcytokineIL-10assaysfirstindicatedexertedCCI-inducedmightattributedactivitiesNeuroprotectiveEffectPainInducedConstrictionInjurySciaticNerveMicetestsInflammationNeuroprotection

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