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Ecology and evolution
Oct, 2018;8 (19): 9793-9802.

Fecal microbiota associated with phytohaemagglutinin-induced immune response in nestlings of a passerine bird.

Jakub Kreisinger, Lucie Schmiedová, Adéla Petrželková, Oldřich Tomášek, Marie Adámková, Romana Michálková, Jean-François Martin, Tomáš Albrecht
Author Information
  1. Jakub Kreisinger: Department of Zoology Faculty of Science Charles University Prague Czech Republic. ORCID
  2. Lucie Schmiedová: Department of Zoology Faculty of Science Charles University Prague Czech Republic.
  3. Adéla Petrželková: Department of Ecology Faculty of Science Charles University Prague Czech Republic.
  4. Oldřich Tomášek: Department of Zoology Faculty of Science Charles University Prague Czech Republic. ORCID
  5. Marie Adámková: Czech Academy of Sciences Institute of Vertebrate Biology Brno Czech Republic.
  6. Romana Michálková: Department of Zoology Faculty of Science Charles University Prague Czech Republic.
  7. Jean-François Martin: Montpellier-SupAgro UMR CBGP Montferrier-sur-Lez France.
  8. Tomáš Albrecht: Department of Zoology Faculty of Science Charles University Prague Czech Republic. ORCID
PMID: 30386575 DOI: 10.1002/ece3.4454

Abstract

The vertebrate gastrointestinal tract is inhabited by a diverse community of bacteria, the so-called gut microbiota (GM). Research on captive mammalian models has revealed tight mutual interactions between immune functions and GM. However, our knowledge of GM versus immune system interactions in wild populations and nonmammalian species remains poor. Here, we focus on the association between GM community structure and immune response measured via the phytohaemagglutinin (PHA) skin swelling test in 12-day-old nestlings of a passerine bird, the barn swallow (). The PHA test, a widely used method in field ecoimmunology, assesses cell-mediated immunity. GM structure was inferred based on high-throughput 16S rRNA sequencing of microbial communities in fecal samples. We did not find any association between PHA response and GM diversity; however, our data revealed that the intensity of PHA response was correlated with differences in GM composition at the whole-community level. Ten bacterial operational taxonomic units corresponding to both putative commensal and pathogens were identified as drivers of the compositional variation. In conclusion, our study suggests existence of GM versus immune system interactions in a free-living nonmammalian species, which corresponds with previous research on captive vertebrates.

Keywords

fitness immunity inflammation metabarcoding microbiome symbiosis

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Journal Article
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