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11 24, 2018;10 (12):

A Novel Highly Divergent Strain of Cell Fusing Agent Virus (CFAV) in Mosquitoes from the Brazilian Amazon Region.

Licia Natal Fernandes, Thaís de Moura Coletti, Fred Julio Costa Monteiro, Marlisson Octavio da Silva Rego, Edcelha Soares D'Athaide Ribeiro, Geovani de Oliveira Ribeiro, Robson Dos Santos Souza Marinho, Shirley Vasconcelos Komninakis, Steven S Witkin, Xutao Deng, Eric Delwart, Ester Cerdeira Sabino, Élcio Leal, Antonio Charlys da Costa
Author Information
  1. Licia Natal Fernandes: Laboratory of Protozoology, Institute of Tropical Medicine, University of São Paulo, São Paulo, SP 05403-000, Brazil. licianatal@usp.br.
  2. Thaís de Moura Coletti: Laboratory of Protozoology, Institute of Tropical Medicine, University of São Paulo, São Paulo, SP 05403-000, Brazil. thaiscoletti@gmail.com.
  3. Fred Julio Costa Monteiro: Laboratory of Vectors, Superintendence for the Health Survaillance of Amapá, Macapá, AP 68905-230, Brazil. fredjulio@gmail.com.
  4. Marlisson Octavio da Silva Rego: Laboratory of Vectors, Superintendence for the Health Survaillance of Amapá, Macapá, AP 68905-230, Brazil. farmarlisson@hotmail.com.
  5. Edcelha Soares D'Athaide Ribeiro: Laboratory of Vectors, Superintendence for the Health Survaillance of Amapá, Macapá, AP 68905-230, Brazil. edcelhamanu@hotmail.com.
  6. Geovani de Oliveira Ribeiro: Institute of Biologycal Sciences, Federal University of Pará, Belém, Pará 66075-000, Brazil. geovanioribeiro@gmail.com.
  7. Robson Dos Santos Souza Marinho: Laboratory of Retrovirology, Federal University of São Paulo, São Paulo, SP 04039-032, Brazil. elcioleal@gmail.com.
  8. Shirley Vasconcelos Komninakis: Laboratory of Retrovirology, Federal University of São Paulo, São Paulo, SP 04039-032, Brazil. skomninakis@yahoo.com.br.
  9. Steven S Witkin: Department of Obstetrics and Gynecology, Weill Cornell Medicine, New York, NY 10065, USA. robsonsantos@id.uff.br.
  10. Xutao Deng: Vitalant Research Institute, 270 Masonic Avenue, San Francisco, CA 94118-4417, USA. xdeng@vitalant.org.
  11. Eric Delwart: Vitalant Research Institute, 270 Masonic Avenue, San Francisco, CA 94118-4417, USA. eric.delwart@ucsf.edu.
  12. Ester Cerdeira Sabino: Institute of Tropical Medicine, University of São Paulo, São Paulo, SP 05403-000, Brazil. witkinss@gmail.com.
  13. Élcio Leal: Institute of Biologycal Sciences, Federal University of Pará, Belém, Pará 66075-000, Brazil. sabinoec@gmail.com.
  14. Antonio Charlys da Costa: Institute of Tropical Medicine, University of São Paulo, São Paulo, SP 05403-000, Brazil. charlysbr@yahoo.com.br.
PMID: 30477235 DOI: 10.3390/v10120666

Abstract

Classical insect-specific flaviviruses (cISFs) have been widely detected in different countries in the last decades. Here, we characterize the near full-length genomes of two cISFs detected in mosquitoes collected in the city of Macapá, state of Amapá, Amazon region of Brazil. A total of 105 pools of female mosquitos were analyzed by next-generation sequencing (NGS). Comparative genomics and phylogenetic analysis identified three strains of cell fusing agent virus (CFAV) and two of Culex flavivirus (CxFV). All sequences were obtained from pools of Culex sp., except for one sequence of CFAV detected in a pool of Aedes aegypti. Both CxFV strains are phylogenetically related to a strain isolated in 2012 in the Southeast region of Brazil. The CFAV strains are the first of this species to be identified in Brazil and one of them is highly divergent from other strains of CFAV that have been detected worldwide. In conclusion, CFAV and CxFV, circulate in mosquitoes in Brazil. One strain of CFAV is highly divergent from others previously described, suggesting that a novel strain of CFAV is present in this region.

Keywords

Amazon region Culex flavivirus Culex sp., Aedes aegypti cell fusing agent virus flavivirus mosquitoes

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Grants

  1. 2016/01735-2/Fundação de Amparo à Pesquisa do Estado de São Paulo
  2. CNPq 400354/2016-0/Conselho Nacional de Desenvolvimento Científico e Tecnológico
  3. 2017/00021-9/Fundação de Amparo à Pesquisa do Estado de São Paulo
  4. FAPESP 2016/18204-0/Fundação de Amparo à Pesquisa do Estado de São Paulo
  5. 250.203.0333/2016/FAPEAP/CNPq PPSUS

MeSH Term

Animals
Brazil
Culicidae
Evolution, Molecular
Flavivirus
Genome, Viral
High-Throughput Nucleotide Sequencing
Phylogeny
Tropical Climate
Viral Nonstructural Proteins

Chemicals

NS5 protein, flavivirus
Viral Nonstructural Proteins
Journal Article Research Support, Non-U.S. Gov't

Word Cloud

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