Hormonal Responses to Infection in Cultivars Differing in Their Pathogen Resistance.
Sylva Prerostova, Petre I Dobrev, Veronika Konradyova, Vojtech Knirsch, Alena Gaudinova, Barbara Kramna, Jan Kazda, Jutta Ludwig-Müller, Radomira Vankova
Author Information
Sylva Prerostova: Institute of Experimental Botany Czech Acad Sci, Laboratory of Hormonal Regulations in Plants, Rozvojova 263, 165 02 Prague 6, Czech Republic. prerostova@ueb.cas.cz. ORCID
Petre I Dobrev: Institute of Experimental Botany Czech Acad Sci, Laboratory of Hormonal Regulations in Plants, Rozvojova 263, 165 02 Prague 6, Czech Republic. dobrev@ueb.cas.cz.
Veronika Konradyova: Faculty of Agrobiology, Food and Natural Resources, Department of Plant Protection, Czech University of Life Sciences Prague, Kamycka 129, 165 00 Prague 6, Czech Republic. konradyova@af.czu.cz. ORCID
Vojtech Knirsch: Institute of Experimental Botany Czech Acad Sci, Laboratory of Hormonal Regulations in Plants, Rozvojova 263, 165 02 Prague 6, Czech Republic. knirsch@ueb.cas.cz.
Alena Gaudinova: Institute of Experimental Botany Czech Acad Sci, Laboratory of Hormonal Regulations in Plants, Rozvojova 263, 165 02 Prague 6, Czech Republic. gaudinova@ueb.cas.cz. ORCID
Barbara Kramna: Institute of Experimental Botany Czech Acad Sci, Laboratory of Hormonal Regulations in Plants, Rozvojova 263, 165 02 Prague 6, Czech Republic. kramna@ueb.cas.cz. ORCID
Jan Kazda: Faculty of Agrobiology, Food and Natural Resources, Department of Plant Protection, Czech University of Life Sciences Prague, Kamycka 129, 165 00 Prague 6, Czech Republic. kazda@af.czu.cz.
Jutta Ludwig-Müller: Dresden, Faculty of Biology, Institute of Botany, Technische Universität, 01062 Dresden, Germany. jutta.ludwig-mueller@tu-dresden.de.
Radomira Vankova: Institute of Experimental Botany Czech Acad Sci, Laboratory of Hormonal Regulations in Plants, Rozvojova 263, 165 02 Prague 6, Czech Republic. vankova@ueb.cas.cz. ORCID
Hormonal dynamics after infection were compared in two cultivars-more resistant SY Alister and more sensitive Hornet, in order to elucidate responses associated with efficient defense. Both cultivars responded to infection by the early transient elevation of active cytokinins (predominantly -zeatin) and auxin indole-3-acetic acid (IAA) in leaves and roots, which was longer in Hornet. Moderate IAA levels in Hornet roots coincided with a high expression of biosynthetic gene (contrary to , , ). Alister had a higher basal level of salicylic acid (SA), and it stimulated its production (via the expression of ()) in roots earlier than Hornet. Gall formation stimulated cytokinin, auxin, and SA levels-with a maximum 22 days after inoculation (dai). SA marker gene expression was the most profound at the time point where gall formation began, in leaves, roots, and especially in galls. Jasmonic acid (JA) was higher in Hornet than in Alister during the whole experiment. To investigate SA and JA function, SA was applied before infection, and twice (before infection and 15 dai), and JA at 15 dai. Double SA application diminished gall formation in Alister, and JA promoted gall formation in both cultivars. Activation of SA/JA pathways reflects the main differences in clubroot resistance.
European Regional Development Fund-Project "Centre for Experimental Plant Biology": grant number CZ.02.1.01/0.0/0.0/16_019/0000738/Ministerstvo Školství, Mládeže a Tělovýchovy
LD15093/Ministerstvo Školství, Mládeže a Tělovýchovy
