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Journal of Zhejiang University. Science. B
2019 Jan.;20 (1): 59-70.

Purification and identification of novel cytotoxic oligopeptides from soft coral Sarcophyton glaucum.

Yixian Quah, Nor Ismaliza Mohd Ismail, Jillian Lean Sim Ooi, Yang Amri Affendi, Fazilah Abd Manan, Lai-Kuan Teh, Fai-Chu Wong, Tsun-Thai Chai
Author Information
  1. Yixian Quah: Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia.
  2. Nor Ismaliza Mohd Ismail: Department of Biological Science, Faculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia.
  3. Jillian Lean Sim Ooi: Department of Geography, Faculty of Arts and Social Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia.
  4. Yang Amri Affendi: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
  5. Fazilah Abd Manan: Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Malaysia.
  6. Lai-Kuan Teh: Centre for Biodiversity Research, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia.
  7. Fai-Chu Wong: Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia.
  8. Tsun-Thai Chai: Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia.
PMID: 30614230 DOI: 10.1631/jzus.B1700586

Abstract

Globally, peptide-based anticancer therapies have drawn much attention. Marine organisms are a reservoir of anticancer peptides that await discovery. In this study, we aimed to identify cytotoxic oligopeptides from Sarcophyton glaucum. Peptides were purified from among the S. glaucum hydrolysates produced by alcalase, chymotrypsin, papain, and trypsin, guided by a methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay on the human cervical cancer (HeLa) cell line for cytotoxicity evaluation. Purification techniques adopted were membrane ultrafiltration, gel filtration chromatography, solid phase extraction (SPE), and reversed-phase high-performance liquid chromatography (RP-HPLC). Purified peptides were identified by de novo peptide sequencing. From papain hydrolysate, three peptide sequences were identified: AGAPGG, AERQ, and RDTQ (428.45, 502.53, and 518.53 Da, respectively). Peptides synthesized from these sequences exhibited cytotoxicity on HeLa cells with median effect concentration (EC) values of 8.6, 4.9, and 5.6 mmol/L, respectively, up to 5.8-fold stronger than the anticancer drug 5-fluorouracil. When tested at their respective EC, AGAPGG, AERQ, and RDTQ showed only 16%, 25%, and 11% cytotoxicity to non-cancerous Hek293 cells, respectively. In conclusion, AERQ, AGAPGG, and RDTQ are promising candidates for future development as peptide-based anticancer drugs.

Keywords

Anticancer therapy Bioactive peptide Cytotoxicity HeLa cells Sarcophyton glaucum Soft coral

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MeSH Term

Amino Acid Sequence
Animals
Anthozoa
Antineoplastic Agents
Chromatography, Gel
Chromatography, High Pressure Liquid
Chromatography, Reverse-Phase
Cytotoxins
Drug Discovery
HEK293 Cells
HeLa Cells
Humans
Hydrolysis
Marine Toxins
Oligopeptides
Solid Phase Extraction
Tandem Mass Spectrometry

Chemicals

Antineoplastic Agents
Cytotoxins
Marine Toxins
Oligopeptides
Journal Article

Word Cloud

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