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01 26, 2019;11 (2):

The Characteristics and Genome Analysis of vB_AviM_AVP, the First Phage Infecting .

Hengyu Xi, Jiaxin Dai, Yigang Tong, Mengjun Cheng, Feiyang Zhao, Hang Fan, Xinwei Li, Ruopeng Cai, Yalu Ji, Changjiang Sun, Xin Feng, Liancheng Lei, Sadeeq Ur Rahman, Wenyu Han, Jingmin Gu
Author Information
  1. Hengyu Xi: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. m15543135579@163.com.
  2. Jiaxin Dai: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. 15204525855@163.com.
  3. Yigang Tong: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China. tong.yigang@gmail.com.
  4. Mengjun Cheng: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. mengjun_c@163.com.
  5. Feiyang Zhao: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China. yangfan10159532@163.com.
  6. Hang Fan: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China. Fanhang11@gmail.com.
  7. Xinwei Li: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. vetboss@163.com.
  8. Ruopeng Cai: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. saycall850@126.com.
  9. Yalu Ji: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. jiyalu120@163.com.
  10. Changjiang Sun: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. schangjiang@126.com.
  11. Xin Feng: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. xinple@163.com.
  12. Liancheng Lei: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. leiliancheng@163.com.
  13. Sadeeq Ur Rahman: College of Veterinary Sciences & Animal Husbandry, Abdul Wali Khan University, Mardan 23200, Pakistan. sadeeq@awkum.edu.pk. ORCID
  14. Wenyu Han: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. hanwy@jlu.edu.cn.
  15. Jingmin Gu: Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China. jingmin0629@163.com. ORCID
PMID: 30691182 DOI: 10.3390/v11020104

Abstract

is an opportunistic pathogen that is clinically associated with various human and animal diseases. In this study, the first identified phage, vB_AviM_AVP (abbreviated as AVP), was isolated and studied. AVP belongs to the family Myoviridae. AVP harbors a double-stranded DNA genome with a length of 133,806 bp and a G + C content of 34.51%. The genome sequence of AVP showed low similarity (<1% identity) to those of other phages, bacteria, or other organisms in the database. Among 165 predicted open reading frames (ORFs), there were only 69 gene products exhibiting similarity (≤65% identity) to proteins of known functions in the database. In addition, the other 36 gene products did not match any viral or prokaryotic sequences in any publicly available database. On the basis of the putative functions of the ORFs, the genome of AVP was divided into three modules: nucleotide metabolism and replication, structural components, and lysis. A phylogenetic analysis of the terminase large subunits and capsid proteins indicated that AVP represents a novel branch of phages. The observed characteristics of AVP indicate that it represents a new class of phages.

Keywords

Aerococcus viridans genome analysis opportunistic pathogen phage

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Grants

  1. 31872505/National Natural Science Foundation of China
  2. 31502103/National Natural Science Foundation of China
  3. 31572553/National Natural Science Foundation of China
  4. 2018YFD0500504/National Key Research and Development Program of China

MeSH Term

Aerococcus
Base Composition
Capsid Proteins
DNA, Viral
Genome, Viral
Myoviridae
Open Reading Frames
Phylogeny
Sequence Analysis, DNA

Chemicals

Capsid Proteins
DNA, Viral
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 2

Word Cloud

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