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The Journal of infectious diseases
08 30, 2019;220 (7): 1099-1108.

Gardnerella vaginalis and Prevotella bivia Trigger Distinct and Overlapping Phenotypes in a Mouse Model of Bacterial Vaginosis.

Nicole M Gilbert, Warren G Lewis, Guocai Li, Dorothy K Sojka, Jean Bernard Lubin, Amanda L Lewis
Author Information
  1. Nicole M Gilbert: Department of Obstetrics and Gynecology, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri.
  2. Warren G Lewis: Department of Molecular Microbiology, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri.
  3. Guocai Li: Department of Molecular Microbiology, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri.
  4. Dorothy K Sojka: Department of Medicine, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri.
  5. Jean Bernard Lubin: Department of Molecular Microbiology, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri.
  6. Amanda L Lewis: Department of Obstetrics and Gynecology, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri.
PMID: 30715405 DOI: 10.1093/infdis/jiy704

Abstract

BACKGROUND: Bacterial vaginosis (BV) is a common imbalance of the vaginal microbiota characterized by overgrowth of diverse Actinobacteria, Firmicutes, and Gram-negative anaerobes. Women with BV are at increased risk of secondary reproductive tract infections and adverse pregnancy outcomes. However, which specific bacteria cause clinical features of BV is unclear.
METHODS: We previously demonstrated that Gardnerella vaginalis could elicit many BV features in mice. In this study, we established a BV model in which we coinfected mice with G. vaginalis and another species commonly found in women with BV: Prevotella bivia.
RESULTS: This coinfection model recapitulates several aspects of human BV, including vaginal sialidase activity (a diagnostic BV feature independently associated with adverse outcomes), epithelial exfoliation, and ascending infection. It is notable that G. vaginalis facilitated uterine infection by P. bivia.
CONCLUSIONS: Taken together, our model provides a framework for advancing our understanding of the role of individual or combinations of BV-associated bacteria in BV pathogenesis.

Keywords

coinfection exfoliation sialidase vagina

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Grants

  1. C06 RR015502/NCRR NIH HHS
  2. K01 DK110225/NIDDK NIH HHS
  3. R01 AI114635/NIAID NIH HHS
  4. R01 AI127554/NIAID NIH HHS

MeSH Term

Animals
Coinfection
Disease Models, Animal
Female
Gardnerella vaginalis
Humans
Mice
Mice, Inbred C57BL
Microbiota
Neuraminidase
Phenotype
Prevotella
RNA, Bacterial
RNA, Ribosomal, 16S
Reverse Transcriptase Polymerase Chain Reaction
Vagina
Vaginosis, Bacterial

Chemicals

RNA, Bacterial
RNA, Ribosomal, 16S
Neuraminidase
Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't
Article has an altmetric score of 1

Word Cloud

Created with Highcharts 10.0.0BVvaginalismodelbiviaBacterialvaginaladverseoutcomesbacteriafeaturesGardnerellamiceGPrevotellacoinfectionsialidaseexfoliationinfectionBACKGROUND:vaginosiscommonimbalancemicrobiotacharacterizedovergrowthdiverseActinobacteriaFirmicutesGram-negativeanaerobesWomenincreasedrisksecondaryreproductivetractinfectionspregnancyHoweverspecificcauseclinicalunclearMETHODS:previouslydemonstratedelicitmanystudyestablishedcoinfectedanotherspeciescommonlyfoundwomenBV:RESULTS:recapitulatesseveralaspectshumanincludingactivitydiagnosticfeatureindependentlyassociatedepithelialascendingnotablefacilitateduterinePCONCLUSIONS:TakentogetherprovidesframeworkadvancingunderstandingroleindividualcombinationsBV-associatedpathogenesisTriggerDistinctOverlappingPhenotypesMouseModelVaginosisvagina

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