Alejandro Villasante: Laboratorio de Biotecnología de Alimentos, Unidad de Alimentos, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Santiago 7830490, Chile. alejandro.villasante@gmail.com.
Carolina Ramírez: Laboratorio de Biotecnología de Alimentos, Unidad de Alimentos, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Santiago 7830490, Chile. carolina.ramirez.saavedra@gmail.com.
Natalia Catalán: Laboratorio de Biotecnología de Alimentos, Unidad de Alimentos, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Santiago 7830490, Chile. nataliabcatalant@gmail.com.
Rafael Opazo: Laboratorio de Biotecnología de Alimentos, Unidad de Alimentos, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Santiago 7830490, Chile. ropazo@inta.uchile.cl.
Patricio Dantagnan: Núcleo de Investigación en Producción Alimentaria, Facultad de Recursos Naturales, Departamento de Ciencias Agropecuarias y Acuícolas, Universidad Católica de Temuco, Temuco 4781312, Chile. dantagna@uct.cl. ORCID
Jaime Romero: Laboratorio de Biotecnología de Alimentos, Unidad de Alimentos, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Santiago 7830490, Chile. jromero@inta.uchile.cl. ORCID
Atlantic salmon () is a carnivorous fish species whose productive performance tends to be suboptimal when fed low-cost carbohydrate rich meals. It is of interest to study the dynamics of gut microbiota communities in salmonids fed high carbohydrate diets since gut microbes are referred to as key players that influence the metabolism and physiology of the host. A study was conducted to determine the effect of feeding a high carbohydrate diet to Atlantic salmon in gut microbiota communities. A medium carbohydrate (15% wheat starch)/medium protein (MC/MP) diet or a high carbohydrate (30% wheat starch)/low protein (HC/LP) diet was fed to triplicate tanks (28 fish each) during four weeks. We conducted an in-depth characterization of the distal intestine digesta microbiota using high-throughput sequencing of the V4 region of the 16S rRNA gene. , and were the major phyla determined in either experimental group. Phylum , class , order and genus were significantly more abundant in fish fed the HC/LP diet compared with fish fed the MC/MP diet. Our study suggests feeding a carbohydrate rich meal to salmon exerts a low impact on the structure of gut microbial communities, affecting mostly low-abundance bacteria capable of metabolizing anaerobically carbohydrates as a major energy-yielding substrate.
