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03 29, 2019;39 (3):

Resveratrol inhibits IL-1β-mediated nucleus pulposus cell apoptosis through regulating the PI3K/Akt pathway.

Yanhai Jiang, Zhijie Xie, Jinying Yu, Lianqiang Fu
Author Information
  1. Yanhai Jiang: Department of Orthopaedics, The Affiliated Weihai Second Hospital of Qingdao University, Weihai 264200, China jiangyanhai1210@163.com. ORCID
  2. Zhijie Xie: Department of Orthopaedics, The Affiliated Weihai Second Hospital of Qingdao University, Weihai 264200, China.
  3. Jinying Yu: Department of Orthopaedics, The Affiliated Weihai Second Hospital of Qingdao University, Weihai 264200, China.
  4. Lianqiang Fu: Department of Orthopaedics, The Affiliated Weihai Second Hospital of Qingdao University, Weihai 264200, China.
PMID: 30867252 DOI: 10.1042/BSR20190043

Abstract

Nucleus pulposus (NP) cell apoptosis is a classical cellular character during intervertebral disc degeneration (IDD). Previous studies have shown that inflammatory cytokine-induced NP cell apoptosis plays an important role in disc degeneration. The present study was aimed to investigate whether resveratrol can suppress IL-1β-mediated NP cell apoptosis and the potential signal transduction pathway. Experimental rat NP cells were treated with culture medium containing IL-1β (20 ng/ml) for 7 days. Control NP cells were cultured in the baseline medium. Resveratrol was added along with culture medium to investigate its effects. The inhibitor LY294002 was used to study the role of the PI3K/Akt pathway. NP cell apoptosis was reflected by the caspase-3 activity, cell apoptosis ratio, and expression of apoptosis-related molecules (Bcl-2, Bax, caspase-3, cleaved caspase-3, and cleaved PARP). Compared with the control NP cells, IL-1β significantly increased caspase-3 activity, NP cell apoptosis ratio and mRNA/protein expression of Bax, caspase-3, cleaved caspase-3 and cleaved PARP, but decreased mRNA expression of Bcl-2. However, resveratrol partly suppressed the effects of IL-1β on those cell apoptosis-related parameters. Further analysis showed that IL-1β significantly decreased activity of the PI3K/Akt pathway whereas resveratrol partly increased activity of the PI3K/Akt pathway in NP cells treated with IL-1β. Additionally, when the inhibitor LY294002 was added along with the resveratrol, its protective effects against IL-1β-induced NP cell apoptosis were attenuated. In conclusion, resveratrol suppresses IL-1β-mediated NP cell apoptosis through activating the PI3K/Akt pathway. Resveratrol may be an effective drug to attenuate inflammatory cytokine-induced disc degenerative changes.

Keywords

IL-1β apoptosis intervertebral disc degeneration nucleus pulposus resveratrol

References

  1. Biosci Rep. 2018 Mar 9;38(2): [PMID: 29439139]
  2. Stem Cells Int. 2017;2017:9843120 [PMID: 29387092]
  3. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2018 Apr 1;32(4):476-483 [PMID: 29806307]
  4. Biochem Soc Trans. 2002 Nov;30(Pt 6):858-64 [PMID: 12440933]
  5. Exp Biol Med (Maywood). 2016 Apr;241(8):848-53 [PMID: 26946533]
  6. Spine (Phila Pa 1976). 2017 Jul 1;42(13):E757-E766 [PMID: 27792110]
  7. Nanomedicine (Lond). 2018 Jun;13(12):1433-1446 [PMID: 29658365]
  8. Mol Nutr Food Res. 2014 Jan;58(1):7-21 [PMID: 23740855]
  9. Biores Open Access. 2018 Feb 01;7(1):2-9 [PMID: 29445584]
  10. Spine (Phila Pa 1976). 2008 Nov 15;33(24):2586-95 [PMID: 19011540]
  11. Spine (Phila Pa 1976). 2000 Feb 15;25(4):487-92 [PMID: 10707396]
  12. Biosci Rep. 2018 Apr 13;38(2): [PMID: 29273676]
  13. J Mol Histol. 2015 Dec;46(6):475-83 [PMID: 26446668]
  14. J Orthop Res. 2017 Jan;35(1):8-22 [PMID: 27104885]
  15. Antioxid Redox Signal. 2001 Dec;3(6):1041-64 [PMID: 11813979]
  16. J Orthop Res. 2017 Jan;35(1):86-92 [PMID: 27035885]
  17. Exp Ther Med. 2015 Dec;10(6):2437-2443 [PMID: 26668654]
  18. Int J Mol Med. 2018 May;41(5):2485-2492 [PMID: 29436588]
  19. Arthritis Res Ther. 2003;5(3):120-30 [PMID: 12723977]
  20. Biochem Biophys Res Commun. 2017 Nov 4;493(1):373-381 [PMID: 28887038]
  21. J Biomech. 2016 May 3;49(7):1214-1220 [PMID: 26924657]
  22. Int J Biol Sci. 2017 Jan 15;13(2):145-156 [PMID: 28255267]
  23. Genet Mol Res. 2015 Oct 16;14(4):12386-93 [PMID: 26505388]
  24. Connect Tissue Res. 2018 Nov;59(6):593-600 [PMID: 29457525]
  25. PeerJ. 2016 Jan 26;4:e1640 [PMID: 26824000]
  26. Int J Mol Med. 2018 Feb;41(2):969-976 [PMID: 29207021]
  27. Int J Mol Med. 2016 Jun;37(6):1439-48 [PMID: 27121482]
  28. J Korean Med Sci. 2013 Jun;28(6):939-45 [PMID: 23772162]
  29. J Cell Physiol. 2019 Mar;234(3):2373-2385 [PMID: 30192008]
  30. Sci Rep. 2017 Feb 17;7:42938 [PMID: 28211497]
  31. Cell Physiol Biochem. 2017;44(1):229-239 [PMID: 29131030]
  32. Med Sci Monit. 2016 Nov 12;22:4312-4322 [PMID: 27847386]
  33. Spine (Phila Pa 1976). 2015 May 1;40(9):E515-24 [PMID: 25646749]
  34. Pain Physician. 2016 Jan;19(1):E215-26 [PMID: 26752489]
  35. Cell Physiol Biochem. 2018;46(2):644-653 [PMID: 29617687]
  36. J Cell Biochem. 2008 Aug 15;104(6):2131-42 [PMID: 18446786]
  37. Ann N Y Acad Sci. 2007 Jan;1095:441-8 [PMID: 17404056]
  38. J Med Food. 2010 Dec;13(6):1415-23 [PMID: 20946021]
  39. Int J Med Sci. 2016 Feb 20;13(3):225-34 [PMID: 26941583]
  40. Arthritis Care Res (Hoboken). 2012 Jul;64(7):1084-8 [PMID: 22337573]
  41. Curr Rheumatol Rep. 2016 Jul;18(7):43 [PMID: 27289467]
  42. Biosci Rep. 2018 Jul 6;38(4): [PMID: 29752339]
  43. Eur Cell Mater. 2015 Sep 21;30:104-16; discussion 116-7 [PMID: 26388614]

MeSH Term

Animals
Antioxidants
Apoptosis
Caspase 3
Cells, Cultured
Female
Interleukin-1beta
Male
Nucleus Pulposus
Phosphatidylinositol 3-Kinases
Proto-Oncogene Proteins c-akt
Proto-Oncogene Proteins c-bcl-2
Rats, Sprague-Dawley
Resveratrol
Signal Transduction

Chemicals

Antioxidants
Interleukin-1beta
Proto-Oncogene Proteins c-bcl-2
Proto-Oncogene Proteins c-akt
Caspase 3
Resveratrol
Journal Article Research Support, Non-U.S. Gov't Retracted Publication
Article has an altmetric score of 2

Word Cloud

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