Genome-wide profiling of mRNA and lncRNA expression in dengue fever and dengue hemorrhagic fever.

Xiao-Lan Zhong, Xiao-Ming Liao, Fei Shen, Hai-Jian Yu, Wen-Sheng Yan, Yun-Fang Zhang, Jia-Jun Ye, Zhi-Ping Lv
Author Information
  1. Xiao-Lan Zhong: Department of Quality Control Huadu Hospital of Southern Medical University & Guangzhou Huadu District People's Hospital China.
  2. Xiao-Ming Liao: Department of Medicine Huadu Hospital of Southern Medical University & Guangzhou Huadu District People's Hospital China.
  3. Fei Shen: Clinical Laboratory Huadu Hospital of Southern Medical University & Guangzhou Huadu District People's Hospital China.
  4. Hai-Jian Yu: Department of Medicine Huadu Hospital of Southern Medical University & Guangzhou Huadu District People's Hospital China.
  5. Wen-Sheng Yan: Department of Medicine Huadu Hospital of Southern Medical University & Guangzhou Huadu District People's Hospital China.
  6. Yun-Fang Zhang: Department of Medicine Huadu Hospital of Southern Medical University & Guangzhou Huadu District People's Hospital China.
  7. Jia-Jun Ye: Department of Medicine Huadu Hospital of Southern Medical University & Guangzhou Huadu District People's Hospital China.
  8. Zhi-Ping Lv: College of Traditional Chinese Medicine Southern Medical University Guangzhou China.

Abstract

Dengue fever (DF) and dengue hemorrhagic fever (DHF) are recurrent diseases that are widespread in the tropics. Here, we identified candidate genes associated with these diseases by performing integrated analyses of DF (GSE51808) and DHF (GSE18090) microarray datasets in the Gene Expression Omnibus (GEO). In all, we identified 7635 differentially expressed genes (DEGs) in DF and 8147 DEGs in DHF as compared to healthy controls ( < 0.05). In addition, we discovered 215 differentially expressed long non-coding RNAs (DElncRNAs) in DF and 225 DElncRNAs in DHF. There were 1256 common DEGs and eight common DElncRNAs in DHF DF, DHF normal control, and DF normal control groups. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that signal transduction (false discovery rate = 8.33E-10), 'toxoplasmosis', and 'protein processing in endoplasmic reticulum' were significantly enriched pathways for common DEGs. We conclude that the ,, and genes may play crucial roles in DF and DHF, and suggest that our findings may facilitate the identification of biomarkers and the development of new drug design strategies for DF and DHF treatment.

Keywords

References

  1. Virol Sin. 2017 Feb;32(1):16-22 [PMID: 27853992]
  2. Clin Infect Dis. 2001 Jan 15;32(2):204-13 [PMID: 11170909]
  3. BMC Genomics. 2009 Aug 27;10:405 [PMID: 19712483]
  4. Diabetes Technol Ther. 2015 Jul;17(7):475-81 [PMID: 25879401]
  5. PLoS One. 2012;7(6):e38202 [PMID: 22675522]
  6. Nat Med. 2004 Dec;10(12 Suppl):S98-109 [PMID: 15577938]
  7. BMC Bioinformatics. 2006 Jul 06;7:335 [PMID: 16824223]
  8. Nat Rev Immunol. 2011 Jul 15;11(8):532-43 [PMID: 21760609]
  9. Sci Rep. 2017 Mar 24;7:45171 [PMID: 28338050]
  10. Genomics. 1999 Jul 15;59(2):161-7 [PMID: 10409427]
  11. PLoS One. 2012;7(10):e48240 [PMID: 23133577]
  12. J Biol Chem. 2011 Jul 8;286(27):23645-51 [PMID: 21566121]
  13. Mem Inst Oswaldo Cruz. 2013 Sep;108(6):755-62 [PMID: 24037198]
  14. Am J Trop Med Hyg. 2010 Sep;83(3):664-71 [PMID: 20810836]
  15. Antiviral Res. 2013 Nov;100(2):500-19 [PMID: 24076358]
  16. PLoS Pathog. 2014 Dec 04;10(12):e1004548 [PMID: 25474532]
  17. Medicine (Baltimore). 2017 Oct;96(42):e8261 [PMID: 29049217]
  18. Dev Dyn. 2004 Jun;230(2):325-34 [PMID: 15162511]
  19. Emerg Infect Dis. 2003 Aug;9(8):1003-6 [PMID: 12967502]
  20. PLoS Negl Trop Dis. 2008 Apr 09;2(4):e215 [PMID: 18398488]
  21. Hum Pathol. 1999 Sep;30(9):1106-10 [PMID: 10492047]
  22. Asian Pac J Cancer Prev. 2014;15(5):2345-51 [PMID: 24716982]
  23. Immunol Today. 1996 May;17(5):214-7 [PMID: 8991382]
  24. Virology. 2001 Mar 30;282(1):141-53 [PMID: 11259197]
  25. Br J Cancer. 2015 Dec 22;113(12):1746 [PMID: 26695556]
  26. Asian Pac J Cancer Prev. 2015;16(7):2793-800 [PMID: 25854364]
  27. J Virol. 2018 Mar 14;92(7): [PMID: 29321322]
  28. Thromb Haemost. 2009 Dec;102(6):1042-9 [PMID: 19967133]

MeSH Term

Biomarkers
Dengue
Gene Expression Profiling
Humans
RNA, Long Noncoding
RNA, Messenger
Severe Dengue

Chemicals

Biomarkers
RNA, Long Noncoding
RNA, Messenger

Word Cloud

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