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Frontiers in plant science
2019;10 219.

An Improved Transformation System for Cowpea ( L. Walp) via Sonication and a Kanamycin-Geneticin Selection Regime.

Bosibori Bett, Stephanie Gollasch, Andy Moore, Robert Harding, Thomas J V Higgins
Author Information
  1. Bosibori Bett: CSIRO Agriculture and Food, Canberra, ACT, Australia.
  2. Stephanie Gollasch: CSIRO Agriculture and Food, Canberra, ACT, Australia.
  3. Andy Moore: CSIRO Agriculture and Food, Canberra, ACT, Australia.
  4. Robert Harding: Centre for Tropical Crops and Biocommodities, Queensland University of Technology, Brisbane, QLD, Australia.
  5. Thomas J V Higgins: CSIRO Agriculture and Food, Canberra, ACT, Australia.
PMID: 30873198 DOI: 10.3389/fpls.2019.00219

Abstract

An improved cowpea transformation method utilizing -mediated gene delivery to explants derived from the cotyledonary nodes of imbibed cowpea seed is described. The explants were regenerated following a sonication procedure and a stringent selection comprising alternating regimes of kanamycin and geneticin. The method was reproducible and led to the recovery of independent fertile transgenic plants in the greenhouse at a level of about one per cent of starting explants. A transgene encoding an insecticidal protein from was used to demonstrate the efficacy of the system.

Keywords

Agrobacterium cowpea transformation cowpeas geneticin-based selection kanamycin-based selection sonication

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Journal Article
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