Efficient Extraction from Mice Feces for NMR Metabolomics Measurements with Special Emphasis on SCFAs.
Adrian Hauser, Philipp Eisenmann, Claudia Muhle-Goll, Burkhard Luy, Andreas Dötsch, Daniela Graf, Pavleta Tzvetkova
Author Information
Adrian Hauser: Karlsruhe Institute of Technology, Institute of Organic Chemistry, Fritz-Haber Weg 6, 76131 Karlsruhe, Germany. adrian.hauser@partner.kit.edu.
Philipp Eisenmann: Karlsruhe Institute of Technology, Institute of Organic Chemistry, Fritz-Haber Weg 6, 76131 Karlsruhe, Germany. philipp-michael.eisenmann@kit.edu.
Claudia Muhle-Goll: Karlsruhe Institute of Technology, Institute for Biological Interfaces-4, Magnetic Resonance, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany. claudia.muhle-goll@kit.edu.
Burkhard Luy: Karlsruhe Institute of Technology, Institute of Organic Chemistry, Fritz-Haber Weg 6, 76131 Karlsruhe, Germany. burkhard.luy@kit.edu. ORCID
Andreas Dötsch: Max Rubner-Institut, Haid-und-Neu-Straße 9, 76131 Karlsruhe, Germany. andreas.doetsch@mri.bund.de.
Pavleta Tzvetkova: Karlsruhe Institute of Technology, Institute for Biological Interfaces-4, Magnetic Resonance, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany. pavleta.tzvetkova@kit.edu. ORCID
Nuclear magnetic resonance (NMR) spectroscopy is one of the most promising methods for use in metabolomics studies as it is able to perform non targeted measurement of metabolites in a quantitative and non-destructive way. Sample preparation of liquid samples like urine or blood serum is comparatively easy in NMR metabolomics, because mainly buffer and chemical shift reference substance are added. For solid samples like feces suitable extraction protocols need to be defined as initial step, where the exact protocol depends on sample type and features. Focusing on short chain fatty acids (SCFAs) in mice feces, we describe here a set of extraction protocols developed with the aim to suppress changes in metabolite composition within 24 h after extraction. Feces are obtained from mice fed on either standard rodent diet or high fat diet. The protocols presented in this manuscript are straightforward for application, and successfully minimize residual bacterial and enzymatic activities. Additionally, they are able to minimize the lipid background originating from the high fat diet.
