Longitudinal proliferation mapping in vivo reveals NADPH oxidase-mediated dampening of Staphylococcus aureus growth rates within neutrophils.
Elena A Seiß, Anna Krone, Pauline Formaglio, Oliver Goldmann, Susanne Engelmann, Burkhart Schraven, Eva Medina, Andreas J Müller
Author Information
Elena A Seiß: Institute of Molecular and Clinical Immunology, Health Campus Immunology Infectiology and Inflammation (GC-I3), Otto-von-Guericke-University, Leipziger Strasse 44, 39120, Magdeburg, Germany. elena.seiss@med.ovgu.de.
Anna Krone: Institute of Molecular and Clinical Immunology, Health Campus Immunology Infectiology and Inflammation (GC-I3), Otto-von-Guericke-University, Leipziger Strasse 44, 39120, Magdeburg, Germany.
Pauline Formaglio: Institute of Molecular and Clinical Immunology, Health Campus Immunology Infectiology and Inflammation (GC-I3), Otto-von-Guericke-University, Leipziger Strasse 44, 39120, Magdeburg, Germany.
Oliver Goldmann: Infection Immunology, Helmholtz Centre for Infection Research, 38124, Braunschweig, Germany.
Susanne Engelmann: Microbial Proteomics, Helmholtz Centre for Infection Research, 38124, Braunschweig, Germany.
Burkhart Schraven: Institute of Molecular and Clinical Immunology, Health Campus Immunology Infectiology and Inflammation (GC-I3), Otto-von-Guericke-University, Leipziger Strasse 44, 39120, Magdeburg, Germany.
Eva Medina: Infection Immunology, Helmholtz Centre for Infection Research, 38124, Braunschweig, Germany.
Andreas J Müller: Institute of Molecular and Clinical Immunology, Health Campus Immunology Infectiology and Inflammation (GC-I3), Otto-von-Guericke-University, Leipziger Strasse 44, 39120, Magdeburg, Germany. andreas.mueller@med.ovgu.de. ORCID
Upon the onset of inflammatory responses, bacterial pathogens are confronted with altered tissue microenvironments which can critically impact on their metabolic activity and growth. Changes in these parameters have however remained difficult to analyze over time, which would be critical to dissect the interplay between the host immune response and pathogen physiology. Here, we established an in vivo biosensor for measuring the growth rates of Staphylococcus aureus (S. aureus) on a single cell-level over days in an ongoing cutaneous infection. Using intravital 2-photon imaging and quantitative fluorescence microscopy, we show that upon neutrophil recruitment to the infection site and bacterial uptake, non-lethal dampening of S. aureus proliferation occurred. This inhibition was supported by NADPH oxidase activity. Therefore, reactive oxygen production contributes to pathogen containment within neutrophils not only by killing S. aureus, but also by restricting the growth rate of the bacterium.
References
PLoS Pathog. 2018 Oct 22;14(10):e1007374
[PMID: 30346994]
Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3746-51
[PMID: 20133586]
Dev Cell. 2018 Mar 12;44(5):542-553
[PMID: 29533770]
