CD13 tethers the IQGAP1-ARF6-EFA6 complex to the plasma membrane to promote ARF6 activation, β1 integrin recycling, and cell migration.
Mallika Ghosh, Robin Lo, Ivan Ivic, Brian Aguilera, Veneta Qendro, Charan Devarakonda, Linda H Shapiro
Author Information
Mallika Ghosh: Center for Vascular Biology, Department of Cell Biology, University of Connecticut School of Medicine, Farmington, CT 06032, USA. mghosh@uchc.edu lshapiro@uchc.edu. ORCID
Robin Lo: Center for Vascular Biology, Department of Cell Biology, University of Connecticut School of Medicine, Farmington, CT 06032, USA.
Ivan Ivic: Center for Vascular Biology, Department of Cell Biology, University of Connecticut School of Medicine, Farmington, CT 06032, USA. ORCID
Brian Aguilera: Center for Vascular Biology, Department of Cell Biology, University of Connecticut School of Medicine, Farmington, CT 06032, USA.
Veneta Qendro: Center for Vascular Biology, Department of Cell Biology, University of Connecticut School of Medicine, Farmington, CT 06032, USA. ORCID
Charan Devarakonda: Center for Vascular Biology, Department of Cell Biology, University of Connecticut School of Medicine, Farmington, CT 06032, USA.
Linda H Shapiro: Center for Vascular Biology, Department of Cell Biology, University of Connecticut School of Medicine, Farmington, CT 06032, USA. mghosh@uchc.edu lshapiro@uchc.edu. ORCID
Cell attachment to the extracellular matrix (ECM) requires a balance between integrin internalization and recycling to the surface that is mediated by numerous proteins, emphasizing the complexity of these processes. Upon ligand binding in various cells, the β1 integrin is internalized, traffics to early endosomes, and is returned to the plasma membrane through recycling endosomes. This trafficking process depends on the cyclical activation and inactivation of small guanosine triphosphatases (GTPases) by their specific guanine exchange factors (GEFs) and their GTPase-activating proteins (GAPs). In this study, we found that the cell surface antigen CD13, a multifunctional transmembrane molecule that regulates cell-cell adhesion and receptor-mediated endocytosis, also promoted cell migration and colocalized with β1 integrin at sites of cell adhesion and at the leading edge. A lack of CD13 resulted in aberrant trafficking of internalized β1 integrin to late endosomes and its ultimate degradation. Our data indicate that CD13 promoted ARF6 GTPase activity by positioning the ARF6-GEF EFA6 at the cell membrane. In migrating cells, a complex containing phosphorylated CD13, IQGAP1, GTP-bound (active) ARF6, and EFA6 at the leading edge promoted the ARF6 GTPase cycling and cell migration. Together, our findings uncover a role for CD13 in the fundamental cellular processes of receptor recycling, regulation of small GTPase activities, cell-ECM interactions, and cell migration.
References
J Immunol. 2012 Jun 1;188(11):5489-99
[PMID: 22544935]
J Cell Sci. 2005 May 15;118(Pt 10):2085-92
[PMID: 15890984]
