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07 29, 2019;9 (1): 10912.

Elucidating the functional role of Mycobacterium smegmatis recX in stress response.

Deepika Prasad, Divya Arora, Vinay Kumar Nandicoori, K Muniyappa
Author Information
  1. Deepika Prasad: Department of Biochemistry, Indian Institute of Science, Bengaluru, 560012, India.
  2. Divya Arora: National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
  3. Vinay Kumar Nandicoori: National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
  4. K Muniyappa: Department of Biochemistry, Indian Institute of Science, Bengaluru, 560012, India. kmbc@iisc.ac.in.
PMID: 31358794 DOI: 10.1038/s41598-019-47312-3

Abstract

The RecX protein has attracted considerable interest because the recX mutants exhibit multiple phenotypes associated with RecA functions. To further our understanding of the functional relationship between recA and recX, the effect of different stress treatments on their expression profiles, cell yield and viability were investigated. A significant correlation was found between the expression of Mycobacterium smegmatis recA and recX genes at different stages of growth, and in response to different stress treatments albeit recX exhibiting lower transcript and protein abundance at the mid-log and stationary phases of the bacterial growth cycle. To ascertain their roles in vivo, a targeted deletion of the recX and recArecX was performed in M. smegmatis. The growth kinetics of these mutant strains and their sensitivity patterns to different stress treatments were assessed relative to the wild-type strain. The deletion of recA affected normal cell growth and survival, while recX deletion showed no significant effect. Interestingly, deletion of both recX and recA genes results in a phenotype that is intermediate between the phenotypes of the ��recA mutant and the wild-type strain. Collectively, these results reveal a previously unrecognized role for M. smegmatis recX and support the notion that it may regulate a subset of the yet unknown genes involved in normal cell growth and DNA-damage repair.

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MeSH Term

Bacterial Proteins
DNA Repair
Gene Expression Regulation, Bacterial
Mycobacterium smegmatis
Rec A Recombinases
Sequence Deletion

Chemicals

Bacterial Proteins
RecX protein, Xanthomonas campestris
Rec A Recombinases
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 1

Word Cloud

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